Novel expression system for Corynebacterium acetoacidophilum and Escherichia coli based on the T7 RNA polymerase-dependent promoter
Novel expression system for Corynebacterium acetoacidophilum and Escherichia coli based on the T7 RNA polymerase-dependent promoter
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DOI：
10.1007/s00253-013-4900-3
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发表时间：
2013-09-01
影响因子：
5
通讯作者：
Deb, Jahar Kanti
中科院分区：
文献类型：
--
作者：
Equbal, Md Javed;Srivastava, Preeti;Deb, Jahar Kanti
工业上重要的棒状杆菌物种即。嗜乙酰酸棒杆菌似乎是重组蛋白生产的替代宿主；尽管做出了许多努力，但迄今为止尚未在棒状杆菌中建立基于启动子的强大系统。这里描述的是基于 T7 启动子的表达系统，该系统以不依赖于外部诱导物的方式在革兰氏阳性嗜乙酰酸梭菌和革兰氏阴性大肠杆菌中发挥作用。这是棒状杆菌 T7 表达系统的第一份报告。此外，它也是现有大肠杆菌 T7 表达系统中的有用补充。
The industrially important species of corynebacteria viz. Corynebacterium acetoacidophilum appear to be alternative hosts for recombinant protein production; despite many efforts, a strong promoter-based system in corynebacteria has not been established so far. Described here is a T7 promoter-based expression system which was functional in both gram-positive C. acetoacidophilum and gram-negative Escherichia coli in an external inducer independent manner. This is the very first report of a T7 expression system for Corynebacterium sp. Also, it is a useful addition in the existing T7 expression systems of E. coli.