HISTOCHEMICAL AND ULTRASTRUCTURAL CHARACTERISTICS OF RAT-BRAIN PERIVASCULAR MAST-CELLS STIMULATED WITH COMPOUND 48/80 AND CARBACHOL
HISTOCHEMICAL AND ULTRASTRUCTURAL CHARACTERISTICS OF RAT-BRAIN PERIVASCULAR MAST-CELLS STIMULATED WITH COMPOUND 48/80 AND CARBACHOL
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DOI：
10.1016/0306-4522(90)90234-u
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发表时间：
1990-01-01
期刊：
影响因子：
3.3
通讯作者：
THEOHARIDES, TC
中科院分区：
文献类型：
--
作者：
DIMITRIADOU, V;LAMBRACHTHALL, M;THEOHARIDES, TC
已知肥大细胞参与过敏反应，其中它们响应免疫球蛋白E和特异性抗原而分泌许多化学物质，最近已定位于中枢神经系统。这些脑肥大细胞的功能仍然是推测性的，因为它们还没有成为任何组合的功能或详细的形态学研究的主题。在这里，它表明，这些细胞主要是血管周围和染色异染与甲苯胺蓝，但红色的阿辛蓝与番红复染，表明它们含有蛋白多糖非常相似的腹膜，但不是粘膜肥大细胞。心内给药的经典肥大细胞促分泌素，化合物48/80，或乙酰胆碱类似物，引起脑肥大细胞的超微结构变化与分泌一致，但没有胞吐作用。然而，已知相同浓度的卡巴胆碱对腹膜肥大细胞没有影响。这些结果表明，脑肥大细胞与血清肥大细胞具有相同的组织化学特征，但对促分泌素的反应性不同，分泌机制也明显不同。它们对神经递质的反应能力及其独特的分泌机制（可能是选择性的）扩大了它们在脑病理生理学中的可能作用。
Mast cells, known for their involvement in allergic reactions where they secrete numerous chemicals in response to immunoglobulin E and specific antigens, have recently been localized in the central nervous system. The function of these brain mast cells has remained speculative as they have not been the subject of any combined functional or detailed morphological studies. Here it is shown that these cells are primarily perivascular and stain metachromatically with Toluidine Blue, but red with Alcian Blue counterstained with Safranin, indicating that they contain proteoglycans quite similar to those of peritoneal, but not mucosal mast cells. Intracardiac administration of the classic mast cell secretagogue, compound 48/80, or the acetylcholine analog, caused ultrastructural changes in brain mast cells consistent with secretion, but without exocytosis. However, it is known that the same concentration of carbachol has no effect on homogeneic peritoneal mast cells. These results indicate that brain mast cells share histochemical characteristics with serosal mast cells, but differ in the reactivity to secretagogues, and apparently in the mechanism of secretion. Their ability to respond to neurotransmitters and their distinct mechanism of secretion, which may be selective, expands their possible role in brain pathophysiology.