CALCITONIN GENE-RELATED PEPTIDE (CGRP) IMMUNOREACTIVITY IN THE NEURO-ENDOCRINE MERKEL CELLS AND NERVE-FIBERS OF PIG AND HUMAN-SKIN
CALCITONIN GENE-RELATED PEPTIDE (CGRP) IMMUNOREACTIVITY IN THE NEURO-ENDOCRINE MERKEL CELLS AND NERVE-FIBERS OF PIG AND HUMAN-SKIN
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DOI：
10.1007/bf00490231
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发表时间：
1989-01-01
期刊：
影响因子：
--
通讯作者：
BEIRAS, A
中科院分区：
文献类型：
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作者：
GARCIACABALLERO, T;GALLEGO, R;BEIRAS, A
采用免疫组化技术研究了猪鼻皮肤和人指尖皮肤中降钙素基因相关肽（CGRP）的存在。两种动物的默克尔细胞和神经纤维均有CGRP免疫反应性。在猪鼻子皮肤中，含有CGRP的默克尔细胞在鼻窦脊表皮尖端和鼻窦毛囊（触须）的外根鞘形成簇状。CGRP免疫染色的人默克尔细胞在腺表皮脊基底层分离或形成小群。在所有病例中，面对相关神经末梢的默克尔细胞一侧的免疫反应性更强烈（CGRP从未阳性）。默克尔细胞的这一部分有最大密度的密核颗粒，这表明CGRP必须储存在这些颗粒中。真皮和皮下可见含有CGRP免疫反应纤维的神经束，血管常被CGRP神经纤维包围。在猪鼻皮肤中，含有CGRP的神经纤维可穿透表皮，以游离末梢终止，在人指尖的迈斯纳小体中可见少量CGRP免疫反应性神经纤维。
The presence of calcitonin gene-related peptide (CGRP) in the skin of pig snout and human fingertip was investigated using immunohistochemical techniques. CGRP immunoreactivity was found in Merkel cells and nerve fibres of both species. In pig snout skin, Merkel cells containing CGRP were seen forming clusters at the tips of rete ridge epidermis and in the exernal root sheath of sinus hair follicles (vibrissae). Human Merkel cells immunostained for CGRP were found isolated or forming small groups in the basal layer of glandular epidermal ridges. In all cases, immunoreactivity was more intense on the side of the Merkel cell facing the associated nerve terminal (which was never positive for CGRP). This part of the Merkel cell has the greatest density of dense-cored granules, suggesting that CGRP must be stored in these granules. Nerve bundles containing CGRP-immunoreactive fibres were found at dermal and hypodermal level, and blood vessels were often surrounded by CGRP nerve fibres. In pig snout skin some nerve fibres containing CGRP penetrated the epidermis and terminated as free endings, and in the human fingertip a small number of CGRP-immunoreactive nerve fibres were seen in Meissner''s corpuscles.