Bovine mitochondrial ribosomes: effect of cations and heterologous dissociation factors on subunit interactions.
Bovine mitochondrial ribosomes: effect of cations and heterologous dissociation factors on subunit interactions.
复制标题
牛线粒体核糖体：阳离子和异源解离因子对亚基相互作用的影响。
DOI：
10.1016/s0006-291x(87)80180-8
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发表时间：
1987
影响因子：
3.1
通讯作者：
Kraus,BL
中科院分区：
文献类型：
--
作者：
Spremulli,L;Kraus,BL
研究了阳离子和核糖体解离因子对牛线粒体核糖体及其亚基之间平衡的影响。与其他核糖体一样，镁离子促进亚基结合，而单价阳离子促进亚基解离。E.ColiIF-3将阻止线粒体28个S和39个S亚基的重新结合。然而，线粒体亚基所需的IF-3浓度至少是细菌亚基所需的5倍。细胞质因子eIF-6在阻止线粒体核糖体亚基结合方面没有检测到的活性。
The effects of cations and ribosome dissociation factors on the equilibrium between the bovine mitochondrial ribosome and its subunits has been investigated. As observed with other ribosomes, Mg2+ions promote subunit association while monovalent cations promote subunit dissociation.E. coliIF-3 will prevent the reassociation of mitochondrial 28 S and 39 S subunits. However, at least 5-fold higher concentrations of IF-3 are required with mitochondrial subunits than are required with bacterial subunits. The cytoplasmic factor eIF-6, has no detectable activity in preventing mitochondrial ribosomal subunit association.