Cloning and embryonic expression of zebrafish neuropilin genes
Cloning and embryonic expression of zebrafish neuropilin genes
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DOI：
10.1016/j.modgep.2004.01.011
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发表时间：
2004-07-01
影响因子：
1.2
通讯作者：
Moens, CB
中科院分区：
文献类型：
--
作者：
Yu, HH;Houart, C;Moens, CB
神经匹林（Neuropilin, Nrp）是一种3类信号的细胞表面受体和血管内皮生长因子中某些肝素形式的细胞表面受体，在神经系统和心血管系统的发育中，在许多生物过程中起作用，包括轴突引导、神经细胞迁移和血管生成。为了了解神经匹林在斑马鱼胚胎发生中的作用，我们克隆了三个斑马鱼神经匹林同源物，nrp1b， nrp2a和nrp2b。基于同源性，斑马鱼的nrp1b和先前克隆的nrp1a与人类nrp1同源，斑马鱼的nrp2a和2b与人类nrp2同源。我们研究了斑马鱼野生型胚胎从体细胞发生开始到受精后48小时这四种神经匹林基因的表达模式。斑马鱼nrp1a在神经管中表达，包括脑端、上皮、后连合轴突和内侧纵束的细胞、后脑神经元、迷走运动神经元和脊髓运动神经元。斑马鱼的鼻腔、下丘脑下方的颅神经嵴细胞（NCC）衍生组织、内皮前体细胞和干尾血管中表达nrp1b。斑马鱼的nrp2a表达于脑端、垂体前叶、动眼肌和滑车运动神经元、沿视上和后连裂的细胞、后脑菱形球1、后脑神经元、颅骨NCCs和硬膜组。斑马鱼的nrp2b表达于端脑、丘脑、下丘脑、骨骺、前后连裂、视后和视上连裂、嗅轴突轨迹、后脑神经元、颅NCCs、体小体和脊髓神经元。(C) 2004 Elsevier B.V.版权所有
Neuropilin (Nrp), a cell surface receptor for class 3 semaphorms and for certain heparin forms of vascular endothelial growth factors, functions in many biological processes including axon guidance, neural cell migration and angiogenesis in the development of the nervous system and the cardiovascular system. To understand the role of neuropilins in zebrafish embryogenesis, we have cloned three zebrafish neuropilin homologues, nrp1b, nrp2a and nrp2b. Based on synteny, zebrafish nrp1b and the previously cloned nrp1a are orthologous to human nrp1, and zebrafish nrp2a and 2b orthologous to human nrp2. We have characterized the expression patterns of these four zebrafish neuropilin genes in wild type embryos from the beginning of somitogenesis to 48 h post-fertilization. Zebrafish nrp1a is expressed in the neural tube including telencephalon, epithalamus, cells along the axonal trajectory of the posterior commissure and the medial longitudinal fascicle, hindbrain neurons, vagus motor neurons and spinal motoneurons. Zebrafish nrp1b is expressed in the nose, the cranial neural crest cell (NCC) derived tissue underlying the hypothalamus, endothelial precursors and the trunk and tail vasculature. Zebrafish nrp2a is expressed in telencephalon, anterior pituitary, oculomotor and trochlear motor neurons, cells along the supra-optic and posterior commissures, hindbrain rhombomere 1, hindbrain neurons, cranial NCCs and sclerotome. Zebrafish nrp2b is expressed in telencephalon, thalamus, hypothalamus, epiphysis, cells along the anterior and posterior commissures, post-optic and supra-optic commissures and the olfactory axonal trajectory, hindbrain neurons, cranial NCCs, somites and spinal cord neurons. (C) 2004 Elsevier B.V. All rights reserved.