AGROBACTERIUM-TUMEFACIENS SUSCEPTIBILITY AND PLANT-REGENERATION OF 10 SOYBEAN GENOTYPES IN MATURITY GROUP-00 TO GROUP-II
AGROBACTERIUM-TUMEFACIENS SUSCEPTIBILITY AND PLANT-REGENERATION OF 10 SOYBEAN GENOTYPES IN MATURITY GROUP-00 TO GROUP-II
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DOI：
10.2135/cropsci1990.0011183x003000020015x
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发表时间：
1990-03-01
期刊：
影响因子：
2.3
通讯作者：
ORF, JH
中科院分区：
文献类型：
--
作者：
DELZER, BW;SOMERS, DA;ORF, JH
农杆菌介导法转化大豆[Glyine max(L.)[MERR.]植物对特定农杆菌菌株对基因类型的敏感性以及从这些基因类型的转化细胞再生植株的能力都会影响植物。本研究的目的是：(I)测定10个成熟组00~II个大豆品种的子叶对根癌农杆菌C58、A208和A281菌株的敏感性；(Ii)测定相同大豆品种的组织培养再生植株的频率。取10个基因型的1日龄无菌苗和高感成熟度IV组品种‘北京’的子叶切取、损伤，接种根癌农杆菌菌株。在所测试的所有大豆基因型中，菌株C58和A208的相对肿瘤形成频率最高，这是一种敏感性指标。大豆品种北京和皮180529感病最严重。从子叶节芽器官发生组织培养和不定芽形成再生植株中鉴定了相同的基因类型。在培养开始后30周内产生成熟植株频率最高的基因型是实验系HHp、‘Evans’、pI 445799、‘Hodgson 78’、‘Corsoy 79’和pI 180529，这些可能是进行全株转化研究的有用基因。
Agrobacterium-mediated transformation of soybean [Glycine max (L.) Merr.] plants is affected by the susceptibility to genotypes to specific Agrobacterium strains and by the ability to regenerate plants from transformed cells of those genotypes. The objectives of this study were to: (i) determine susceptibility of cotyledons from 10 Maturity Group 00 to II soybean genotypes to strains C58, A208, and A281 of A. tumefaciens and, (ii) determine the frequency of plant regeneration from tissue cultures of the same soybean genotypes. Cotyledons from 1-d-old axenic seedlings of the 10 genotypes and ''Peking'' a highly susceptible Maturity Group IV cultivar, were excised, wounded, and inoculated with the A. tumefaciens strains. The relative frequency of tumor formation, an indicator of susceptibility, was greatest for strains C58 and A208 on all soybean genotypes tested. The soybean genotypes Peking and PI 180529 were most susceptible. The same genotypes were evaluated from shoot organogenic tissue culture initiation from the cotyledonary node and plant regeneration via adventitious shoot formation. The genotypes producing the highest frequency of mature plants during 30 weeks after culture initiation were Experimental Line HHP, ''Evans'', PI 445799, ''Hodgson 78'', ''Corsoy 79'', and PI 180529, These may be useful genotypes for whole plant transformation studies.