Alkalilacustris brevis gen. nov., sp. nov., isolated from a soda lake
Alkalilacustris brevis gen. nov., sp. nov., isolated from a soda lake
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短碱藻属
DOI：
10.1099/ijsem.0.003373
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发表时间：
2019
影响因子：
2.8
通讯作者：
Wu Min
中科院分区：
文献类型：
--
作者：
Zhang Ran;Ju Zhao;Han Shuaibo;Hou Xinjun;Yu Yanghuan;Zhang Xinqi;Ye Yanghui;Sun Cong;Zhang Guishan;Wu Min
从吉林省碱湖水样中分离到一株革兰氏阴性、需氧、无色、短杆状的细菌，编号为34079 T。菌株34079 T在10-50 °C（最适，35 °C），pH 7-10（最适，pH 8.0-8.5）下生长。生长所需的NaCl浓度范围为1- 10.0%（w/v），最佳浓度为2.5- 4%（w/v）。 化学分类学分析表明，唯一的呼吸醌是Q-10。主要的细胞脂肪酸（> 5%）是特征8（C18：1ω 7 c/C18：1ω6c）和C16：0的总和。 主要极性脂质包括二磷脂酰甘油、磷脂酰甘油、磷脂酰胆碱、3种未鉴定的氨基脂质、1种未鉴定的氨基磷酸糖脂、1种磷酸糖脂、1种未鉴定的糖脂、3种未鉴定的磷脂和2种未鉴定的脂质。DNA G+C含量为65.6mol%。基于16 S rRNA基因序列的系统发育分析表明，菌株34079 T与聚集副红杆菌（Pararhodobacter aggregans）的同源性最高，为96.1%，其次为红球藻（RhodobacabogoriensisDSM 18756 T）（95.7%）和玫瑰花球藻（RoseibacaekhonensisDSM 11469 T）（94.7%）。 与先前描述的分类群不同的生物化学、化学分类和系统发育差异支持菌株34079 T代表一个新属的新种，该新属的名称为Alkalilacustris brevisgen。十一月，sp. nov.的报道。模式菌株为34079 T（=KCTC 62428 T =MCCC 1 K 03493 T）。
A Gram-stain-negative, aerobic, non-pigmented and short-rod-shaped bacterium, designated 34079T, was isolated from a water sample of a soda lake in Jilin, a province of China. Strain 34079Tgrew at 10–50 °C (optimum, 35 °C), pH 7–10 (optimum, pH 8.0–8.5). NaCl was required for growth at the concentration range 1–10.0 % (w/v), with an optimum at 2.5–4 % (w/v). Chemotaxonomic analysis indicated that the sole respiratory quinone was Q-10. The predominant cellular fatty acids (>5 %) were summed feature 8 (C18 : 1ω7c/C18 : 1ω6c) and C16 : 0. The major polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, three unidentified amino lipids, one unidentified amino phosphoglycolipid, one phosphoglycolipid, one unidentified glycolipid, three unidentified phospholipids and two unidentified lipids. The DNA G+C content was 65.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 34079Tformed a distinct lineage in the clade of the family ‘Rhodobacteraceae’ with the highest sequence similarity of 96.1 % toPararhodobacter aggregans, followed byRhodobaca bogoriensisDSM 18756T(95.7 %) andRoseibaca ekhonensisDSM 11469T(94.7 %). The distinct biochemical, chemotaxonomic and phylogenetic differences from the previously described taxa supported that strain 34079Trepresents a novel species of a new genus, for which the nameAlkalilacustris brevisgen. nov., sp. nov. is proposed. The type strain is 34079T(=KCTC 62428T=MCCC 1K03493T).