Amplification of growth factor receptor genes and DNA ploidy pattern in the progression of gastric cancer
Amplification of growth factor receptor genes and DNA ploidy pattern in the progression of gastric cancer
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DOI：
10.1007/s004280050115
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发表时间：
1997-12-01
期刊：
影响因子：
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通讯作者：
Hattori, T
中科院分区：
文献类型：
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作者：
Tsujimoto, H;Sugihara, H;Hattori, T
为了研究胃癌中癌基因扩增的背景，我们检测了癌基因扩增的发生与DNA倍体模式的相关性。应用Southern blot分析了57例原发性胃癌中c-erbB、c-erbB-2、c-met和K-sam基因的扩增情况，并采用静态细胞荧光法和流式细胞术测定了DNA倍体模式。II例癌中检测到癌基因扩增，其中晚期胃癌10例，早期分化型1例。c-erbB-2和K-sam基因的扩增分别只在分化型和未分化型癌症中被发现。在11例癌症中，5例为dna二倍体，6例为dna非整倍体。11例癌基因扩增的肿瘤均含有多倍体细胞群（多倍体），而非多倍体的肿瘤均未出现癌基因扩增。在分化型癌症中，多倍体的发生率在早期和晚期都很高，而在未分化型癌症中，多倍体的发生率在早期较低，但在晚期明显较高。结果表明，生长因子受体基因的扩增与多倍体的存在密切相关，而与任何不同的茎系dna -倍体模式无关。癌基因扩增的时间进程和扩增的基因种类在分化型和未分化型胃癌中可能存在差异。
To study the background of oncogene amplification in gastric cancers, we examined the correlation between occurrence of oncogene amplification and DNA ploidy pattern. In 57 primary gastric cancers, amplifications of c-erbB, c-erbB-2, c-met and K-sam genes were investigated by Southern blot analysis, and the DNA ploidy pattern was determined by static cytofluorometry and by flow cytometry. Oncogene amplification was detected in II cancers, 10 of which were advanced gastric cancers and 1 was an early differentiated type. The amplification of c-erbB-2 and K-sam genes was found exclusively in differentiated-and undifferentiated-type cancers, respectively. Of the 11 cancers, 5 were DNA-diploid and 6 were DNA-aneuploid. All the 11 tumours with oncogene amplification contained polyploid cell populations (polyploidy), whereas none of the tumours without polyploidy showed oncogene amplification. In differentiated-type cancers the incidence of polyploidy was high in both early and advanced stages, while in undifferentiated-type cancers it was low in early stages but significantly higher in advanced stages. It was thus shown that amplification of growth factor receptor genes is closely related to the presence of polyploidy, irrespective of any different stemline DNA-ploidy mode. The time-course of oncogene amplification and kinds of genes amplified may differ between differentiated-and undifferentiated-type gastric cancers.