Effects of Maternal Vitamin D3 Supplementation on Offspring Epigenetic Clock of Gestational Age at Birth: A Post-hoc Analysis of a Randomized Controlled Trial
Effects of Maternal Vitamin D3 Supplementation on Offspring Epigenetic Clock of Gestational Age at Birth: A Post-hoc Analysis of a Randomized Controlled Trial
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DOI：
10.1080/15592294.2020.1734148
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发表时间：
2020-03-03
期刊：
影响因子：
3.7
通讯作者：
Zhu, Haidong
中科院分区：
文献类型：
--
作者：
Chen, Li;Wagner, Carol L.;Zhu, Haidong
维生素D可能有益于人类的健康衰老。我们以前发现，维生素D补充剂可能会减缓年轻非洲裔美国人的表观遗传衰老。我们测试了为多种族人群中的新生儿开发的新表观遗传时钟，并测试了补充维生素D会减缓新生儿表观遗传胎龄加速（GAA）的假设。在一项随机对照试验（RCT）中，92名孕妇（年龄29.6 ± 4.8岁; 21%为非洲裔美国人，28%为西班牙裔）在怀孕期间随机接受4000 IU/天维生素D-3或安慰剂，以及含400 IU维生素D-3的产前维生素。在Illumina Infinium MethylationEPIC Beadchip上进行脐带血全基因组甲基化分析。根据Knight和Bohlin开发的两种计算方法计算DNA甲基化胎龄。在安慰剂组中，通过骑士钟和柏林钟计算的DNA甲基化胎龄与胎龄高度相关（相关系数分别为0.88，p < 0.001）。GAA与较高的出生体重相关（p = 0.039）。在整个队列中，维生素D-3补充与GAA无关（p > 0.05）。然而，在非裔美国人参与者中，维生素D-3补充剂降低了骑士钟（β =-0.89，p = 0.047）和Bohlin钟（β =-0.71，p = 0.005）的GAA。补充维生素D-3可能会减缓非裔美国新生儿的表观遗传妊娠衰老过程。需要长期的随访研究来确定表观遗传年龄加速在后代生长发育中的作用。
Vitamin D could be beneficial for healthy ageing in humans. We previously found that vitamin D supplementation may slow down epigenetic ageing in young African American adults. We tested new epigenetic clocks developed for neonates among a multiethnic population, and tested the hypothesis that vitamin D supplementation would slow down the epigenetic gestational age acceleration (GAA) in newborn babies. Ninety-two pregnant women (aged 29.6 +/- 4.8 y; 21% African Americans, 28% Hispanics) were randomized to receive 4000 IU/day vitamin D-3 or placebo, plus prenatal vitamins containing 400 IU vitamin D-3 during pregnancy in a randomized controlled trial (RCT). Cord blood genome-wide methylation analysis was performed on the Illumina Infinium MethylationEPIC Beadchip. DNA methylation gestational age was calculated based on two calculations developed by Knight and Bohlin. DNA methylation gestational ages calculated by Knight's clock and Bohlin' clock were highly correlated with the gestational age in the placebo group (correlation coefficients = 0.88, p s< 0.001, respectively). GAA was associated with higher birth weight (p = 0.039). In the entire cohort, vitamin D-3 supplementation was not associated with GAA (p > 0.05). However, vitamin D-3 supplementation decreased GAA by both Knight's clock (beta = -0.89, p = 0.047) and Bohlin's clock (beta = -0.71, p = 0.005) in the African American participants. Vitamin D-3 supplementation may slow down the epigenetic gestational ageing process in African American neonates. Long-term follow-up studies are warranted to determine the role of epigenetic age acceleration in the growth and development of offspring.