Measurement of particle size distribution in mammalian cells in vitro by use of polarized light spectroscopy.
Measurement of particle size distribution in mammalian cells in vitro by use of polarized light spectroscopy.
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DOI：
10.1364/ao.43.001296
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发表时间：
2004-02
期刊：
影响因子：
1.9
通讯作者：
M. Bartlett;George Huang;L. Larcom;Huabei Jiang
中科院分区：
文献类型：
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作者：
M. Bartlett;George Huang;L. Larcom;Huabei Jiang
我们证明了在体外测量细胞内部结构的粒度分布（PSD）的可行性。我们使用偏振光光谱探测哺乳动物乳腺癌（MCF7）和宫颈癌（Siha）细胞的内部形态。我们发现，绘图的最小平方误差与散射体的大小提供了深入了解细胞散射。一个非线性优化方案被用来确定PSD迭代。结果表明，2微米颗粒（可能是线粒体）对散射贡献最大。其他亚细胞结构，如核仁和细胞核，也可能起重要作用。我们重建PSD的线粒体，通过光学显微镜验证。我们还证明了PSD的角度依赖性。
We demonstrate the feasibility of measuring the particle size distribution (PSD) of internal cell structures in vitro. We use polarized light spectroscopy to probe the internal morphology of mammalian breast cancer (MCF7) and cervical cancer (Siha) cells. We find that graphing the least-squared error versus the scatterer size provides insight into cell scattering. A nonlinear optimization scheme is used to determine the PSD iteratively. The results suggest that 2-microm particles (possibly the mitochondria) contribute most to the scattering. Other subcellular structures, such as the nucleoli and the nucleus, may also contribute significantly. We reconstruct the PSD of the mitochondria, as verified by optical microscopy. We also demonstrate the angle dependence of the PSD.