EFFECT OF INTRAVENOUS INJECTION OF OXIDIZED METHYL ESTERS OF UNSATURATED FATTY ACIDS ON CHICK ENCEPHALOMALACIA
EFFECT OF INTRAVENOUS INJECTION OF OXIDIZED METHYL ESTERS OF UNSATURATED FATTY ACIDS ON CHICK ENCEPHALOMALACIA
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DOI：
10.3181/00379727-105-26093
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发表时间：
1960-01-01
影响因子：
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通讯作者：
KUMMEROW, FA
中科院分区：
文献类型：
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作者：
NISHIDA, T;TSUCHIYAMA, H;KUMMEROW, FA
将各组雄性雏鸡在含有不同水平的玉米油和大豆（Archer Daniels米德兰纯化）蛋白的纯化饮食上饲养一周，并注射乳化在1 ml血清中的10 mg亚油酸甲酯的氢过氧化物。注射后1-5小时，雏鸡出现小脑疾病。然而，在保持相同饮食但补充8 mg% [α]-生育酚的禽类中注射后未观察到小脑疾病。注射还原氢过氧化物、12-氧代-顺式-9-十八烷酸甲酯或新鲜亚油酸甲酯未引起小脑疾病，表明仅脂氢过氧化物引发症状。注射脂过氧化氢诱导的小脑疾病的严重程度受饮食预处理的影响很大。高蛋白、高脂肪、维生素E缺乏的雏鸡小脑被注射脂过氧化氢损伤的范围比正常雏鸡小脑更广。这些数据表明，营养性脑软化症可能是由体内脂氢过氧化物的积累引起的，尽管脂氢过氧化物的水平仍然不可测量。
Groups of male chicks were kept for one week on purified diets, which contained various levels of corn oil and soy (Archer Daniels Midland purified) protein, and injected with 10 mg of the hydroperoxide of methyl linoleate emulsified in 1 ml of serum. Cerebellar disorders developed in chicks 1-5 hours after the injection. However, no cerebellar disorders were noted after injection in birds kept on an identical diet, but supplemented with 8 mg% [alpha]-tocopherol. The injection of reduced hydroperoxide, methyl 12-oxo-cis-9-octadeconate or fresh methyl linoleate caused no cerebellar disorders, indicating only lipohydroperoxide initiated symptoms. The severity of cerebellar disorders induced by the injection of lipohydroperoxide was greatly influenced by dietary pretreatment. The cerebellum of chicks which had been kept on a high protein, high fat, vitamin E deficient diet were damaged more extensively by the injection of lipohydroperoxide than the cerebellum of chicks kept on adequate diets. These data suggest that nutritional encephalomalacia may be initiated by the accumulation of sufficient, though still immeasurable, levels of lipohydroperoxide in vivo.