Up-regulation of peripherin is associated with alterations in synaptic plasticity in CA1 and CA3 regions of hippocampus.
Up-regulation of peripherin is associated with alterations in synaptic plasticity in CA1 and CA3 regions of hippocampus.
复制标题
外周蛋白的上调与海马 CA1 和 CA3 区域突触可塑性的改变有关。
DOI：
10.1016/j.nbd.2004.10.009
复制
发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Krnjevic,Kresimir
中科院分区：
文献类型：
--
作者：
Kriz,Jasna;Beaulieu,Jean-Martin;Julien,Jean-Pierre;Krnjevic,Kresimir
Peripherin是一种III型中间丝蛋白，通常在大多数脑神经元中检测不到。在这里，我们报告了一个类似的模式，外周蛋白的表达在两个小鼠的大脑中的全身注射红藻氨酸（KA）和外周蛋白转基因小鼠（每鼠）过表达正常外周蛋白基因下自己的启动子。双免疫荧光标记显示，部分共同定位的外周蛋白与微管相关蛋白MAP 2，但不与神经丝蛋白。电生理学研究显示，Per小鼠的突触可塑性显著改变：在CA 1中，Per切片的长时程增强（LTP）降低（+29 ± 2.0%，WT中为+58 ± 5.4%）;而在CA 3中，Per的LTP增加（+63 ± 3.5% vs. +43 ± 2.4.0%）。在Per小鼠的海马中，MAP 2的水平降低，但突触素和PSD 95保持不变。这些有趣的发现表明外周蛋白在海马突触可塑性改变中的作用。
Peripherin is a type III intermediate filament protein normally undetectable in most brain neurons. Here, we report a similar pattern of peripherin expression in the brains of both mice treated with systemic injections of kainic acid (KA) and in peripherin transgenic mice (Per mice) over-expressing the normal peripherin gene under its own promoter. Double-immunofluorescence labeling revealed a partial co-localization of peripherin with the microtubule-associated protein MAP2, but not with neurofilament proteins. Electrophysiological studies revealed that synaptic plasticity was markedly altered in Per mice: in CA1, long-term potentiation (LTP) was decreased in Per slices (+29 ± 2.0%, vs. +58 ± 5.4%, in WT); while in CA3, LTP was increased in Per (+63 ± 3.5% vs. +43 ± 2.4.0%). In the hippocampus of Per mice, the levels of MAP2 were decreased, though synaptophysin and PSD95 remained unchanged. These intriguing findings suggest a role of peripherin in the alteration of hippocampal synaptic plasticity.