New immunosensor for Lactoferrin determination in human milk and several pharmaceutical dairy milk products recommended for the unweaned diet
New immunosensor for Lactoferrin determination in human milk and several pharmaceutical dairy milk products recommended for the unweaned diet
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DOI：
10.1016/j.jpba.2007.12.010
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发表时间：
2008-09-29
影响因子：
3.4
通讯作者：
Tomassetti, Mauro
中科院分区：
文献类型：
--
作者：
Campanella, Luigi;Martini, Elisabetta;Tomassetti, Mauro
对乳铁蛋白免疫传感器的研制进行了深入的研究。此外，使用两种不同的竞争性程序进行乳铁蛋白测定，其中抗原（乳铁蛋白）或抗体（抗乳铁蛋白）分别使用生物素化过程与辣根过氧化物酶缀合。乳铁蛋白的生物素化和随后用于免疫传感器测量的竞争程序是准备好的。实施了三种不同类型的免疫传感器，在所有情况下使用过氧化物酶作为标记物和过氧化氢作为底物，但可替代地使用以下传感器之一作为换能器：（i）用于H2O2的电流电极，（ii）Clark电极和（iii）碘化物电极。在优化“竞争性”测量程序和传感器后，新型乳铁蛋白免疫传感器被用于测定人乳和不同类型的奶粉或其他乳制品中的乳铁蛋白含量，这些奶粉或乳制品专门在药店生产和销售，用于喂养出生后几个月内未断奶的儿童。(C)2007 Elsevier B.V.保留所有权利。
Thorough research was carried out on Lactoferrin immunosensor development. Furthermore, two different competitive procedures were used for Lactoferrin determination, in which either the antigen (Lactoferrin) or the antibody (anti -Lactoferrin) was, respectively, conjugated with horseradish peroxidase enzyme using a biotinylation process. The biotinylation of Lactoferrin and the subsequently used competition procedure for the immunosensor measurement were to get ready. Three different kinds of immunosensors were implemented, in all cases using the peroxidase enzyme as marker and hydrogen peroxide as substrate, but alternatively using as transducers one of the following sensors: (i) an amperometric electrode for H2O2, (ii) a Clark electrode and (iii) an iodide electrode. After optimizing the "competitive" measurement procedures and the transducer, the new Lactoferrin immunosensor was used for the determination of Lactoferrin content in human milk and in different types of dried milks or other dairy products, specifically produced and sold in chemist's shops to feed unweaned children in the first few months of life. (C) 2007 Elsevier B.V. All rights reserved.