Microarray analyses of otospheres derived from the cochlea in the inner ear identify putative transcription factors that regulate the characteristics of otospheres.
Microarray analyses of otospheres derived from the cochlea in the inner ear identify putative transcription factors that regulate the characteristics of otospheres.
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DOI：
10.1371/journal.pone.0179901
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Saito MK
中科院分区：
文献类型：
--
作者：
Iki T;Tanaka M;Kitajiri SI;Kita T;Kawasaki Y;Mizukoshi A;Fujibuchi W;Nakagawa T;Nakahata T;Ito J;Omori K;Saito MK
各种组织都有组织特异性的干/祖细胞，包括内耳。通过球体形成实验，可以从分化的细胞中分离出内耳的干/祖细胞，形成所谓的耳球。虽然最近的研究在一定程度上证明了耳球的特征，但这些细胞的大多数特征尚不清楚。在这篇报道中，我们描述了用来自新生小鼠内耳的耳球基因芯片进行转录组分析的结果，以阐明耳干/祖细胞的基因表达谱。耳球和胚胎干细胞之间存在共同的转录因子，这可能是由于耳球的干性所致。与耳蜗感觉上皮相比，耳球具有与耳蜗相同的特征，尽管已鉴定出几个耳球特有的转录因子。这些转录因子被认为对维持耳球的特性是必不可少的，并且似乎是促进细胞直接转化为耳干/祖细胞的候选基因。
Various tissues possess tissue-specific stem/progenitor cells, including the inner ears. Stem/progenitor cells of the inner ear can be isolated as so-called otospheres from differentiated cells using a sphere forming assay. Although recent studies have demonstrated the characteristics of otospheres to some extent, most of the features of these cells are unknown. In this report, we describe the findings of transcriptome analyses with a cDNA microarray of otospheres derived from the cochleae of the inner ears of neonatal mice in order to clarify the gene expression profile of otic stem/progenitor cells. There were common transcription factors between otospheres and embryonic stem cells, which were supposed to be due to the stemness of otospheres. In comparison with the cochlear sensory epithelium, the otospheres shared characteristics with the cochlea, although several transcription factors specific for otospheres were identified. These transcription factors are expected to be essential for maintaining the characteristics of otospheres, and appear to be candidate genes that promote the direct conversion of cells into otic stem/progenitor cells.