Colorimetric Sensing of Dopamine Based on Peroxidase-Like Activity of Gold Nanoparticles
Colorimetric Sensing of Dopamine Based on Peroxidase-Like Activity of Gold Nanoparticles
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基于金纳米粒子的过氧化物酶样活性的多巴胺比色传感
DOI：
10.1134/s1061934819070049
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发表时间：
2019-07-01
影响因子：
1.1
通讯作者：
Jiang, Wanquan
中科院分区：
文献类型：
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作者：
Jiang, Cuifeng;Pang, Shaoping;Jiang, Wanquan
由于多巴胺在许多生理过程中起着重要作用，因此发展一种简单、无标记的多巴胺比色传感检测方法迫在眉睫。本文基于裸金纳米粒子（AuNPs）对过氧化物酶样活性的增强作用，提出了一种无标记比色法测定多巴胺。该测定是基于质子化多巴胺和金原子之间的特定相互作用，通过邻苯二酚基团，诱导聚集的金纳米颗粒，并显着增强其过氧化物酶样活性。用H_2O_2与3，3 ′，5，5 ′-四甲基联苯胺的显色反应评价了催化剂的催化活性。并对催化剂活性增强的机理进行了讨论。多巴胺的检测限为25 nM（紫外-可见光谱法）和0.21 μM（肉眼法）。整个检测过程可在15 min内完成。该传感器简单、快速、成本低、灵敏度高。这些优点使该传感器成为测定水样中多巴胺的有力工具。
Development of a simple and label-free colorimetric sensing assay for dopamine is urgent, because of the important roles dopamine plays in many physiological processes. Herein, a label-free colorimetric method for determination of dopamine has been proposed based on the enhancement of the peroxidase-like activity of bare gold nanoparticles (AuNPs). The assay is based on specific interactions between protonated dopamine and gold atoms through the catechol group, which induces aggregation of AuNPs and significantly enhances their peroxidase-like activity. The catalytic activity is evaluated by chromogenic reaction of 3,3',5,5'-tetramethylbenzidine with H2O2. The mechanism for enhancement of catalytic activity is discussed. The detection limit of dopamine was 25 nM by virtue of UV-Vis spectroscopy, and 0.21 μM by naked eye. The whole detection procedure can be completed in 15 min. The proposed sensor is simple, fast, cost-effective and sensitive. These advantages make this sensor a powerful tool for the determination of dopamine in water samples.