New valve and bonding designs for microfluidic biochips containing proteins
New valve and bonding designs for microfluidic biochips containing proteins
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DOI：
10.1021/ac0615798
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发表时间：
2007-02-01
影响因子：
7.4
通讯作者：
Lee, L. James
中科院分区：
文献类型：
--
作者：
Lu, Chunmeng;Xie, Yubing;Lee, L. James
微流控生物芯片设计和制造中的两个主要问题是通道表面的蛋白质结合和器件组装过程中的蛋白质变性。在本文中，我们描述了解决这些问题的新方法。一种基于超疏水概念的“鱼骨式”微阀设计被开发出来，用于在芯片表面需要蛋白质阻隔以防止非特异性结合的应用中取代毛细管阀。我们的实验结果表明，该阀门在类似CD的酶联免疫吸附试验装置中运行良好。含有预载蛋白质的生物芯片的包装也是一项具有挑战性的任务，因为传统的密封方法通常需要使用高温、电压或对蛋白质活性有害的有机溶剂。使用二氧化碳气体促进聚合物分子在器件表面附近的扩散，可以在低温和低压下实现良好的粘接。这种键合方式对键合后预载蛋白质的活性影响不大。
Two major concerns in the design and fabrication of microfluidic biochips are protein binding on the channel surface and protein denaturing during device assembly. In this paper, we describe new methods to solve these problems. A "fishbone" microvalve design based on the concept of superhydrophobicity was developed to replace the capillary valve in applications where the chip surface requires protein blocking to prevent nonspecific binding. Our experimental results show that the valve functions well in a CD-like ELISA device. The packaging of biochips containing pre-loaded proteins is also a challenging task since conventional sealing methods often require the use of high temperatures, electric voltages, or organic solvents that are detrimental to the protein activity. Using CO2 gas to enhance the diffusion of polymer molecules near the device surface can result in good bonding at low temperatures and low pressure. This bonding method has little influence on the activity of the pre-loaded proteins after bonding.