Substructure of inner dynein arms, radial spokes, and the central pair/projection complex of cilia and flagella.
Substructure of inner dynein arms, radial spokes, and the central pair/projection complex of cilia and flagella.
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DOI：
10.1083/jcb.100.6.2008
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发表时间：
1985-06
期刊：
影响因子：
--
通讯作者：
Heuser JE
中科院分区：
文献类型：
--
作者：
Goodenough UW;Heuser JE
我们分析了衣藻轴突内部组成部分的亚结构——内动力蛋白臂、径向辐条和中央对/投影复合体。四膜虫，强心虫和记忆虫使用速冻，深蚀刻技术。内臂在整个分子组织中与外臂相似，但它们在微管上的配置不同，并且具有两种不同的形态-具有两个头的二联体和具有三个头的三联体。二极体与辐条S3和S2相连；三合会与S1有关。辐条形成一个三开始右手螺旋与288纳米上升；中间的一对做一个浅的左旋。辐条头显示由四个主要亚单位组成；两个绑定到辐条轴和两个绑定到一对中心鞘凸出。
The substructure of the components of the axoneme interior--the inner dynein arms, the radial spokes, and the central pair/projection complex- -was analyzed for Chlamydomonas. Tetrahymena, Strongelocentrotus, and Mnemiopsis using the quick-freeze, deep-etch technique. The inner arms are shown to resemble the outer arms in overall molecular organization, but they are disposed differently on the microtubule and have two distinct morphologies--dyads with two heads and triads with three. The dyads associate with spokes S3 and S2; the triads associate with S1. The spokes form a three-start right-handed helix with a 288-nm rise; the central pair makes a shallow left-handed twist. The spoke heads are shown to be made up of four major subunits; two bind to the spoke shaft and two bind to a pair of central-sheath projections.