ONCOGENICITY OF BK VIRUS FOR IMMUNOSUPPRESSED HAMSTERS
ONCOGENICITY OF BK VIRUS FOR IMMUNOSUPPRESSED HAMSTERS
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DOI：
10.1007/bf01318078
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发表时间：
1982-01-01
影响因子：
2.7
通讯作者：
BARBANTIBRODANO, G
中科院分区：
文献类型：
--
作者：
CORALLINI, A;ALTAVILLA, G;BARBANTIBRODANO, G
通过静脉内接种BK病毒（BKV）在用抗淋巴细胞血清或醋酸甲泼尼龙单独或与γ-葡聚糖联合免疫抑制的3周龄叙利亚金黄仓鼠中诱导肿瘤。辐射（60 Co）。诱发的肿瘤有室管膜瘤、胰岛癌、淋巴瘤、骨肉瘤、未分化肉瘤、肾癌和肾盂癌、嗜铬细胞瘤和血管肉瘤。高水平的胰岛素和胰高血糖素和改变的葡萄糖浓度在胰岛肿瘤动物的血液中检测到。在仓鼠血清中未检测到BKV肿瘤抗原（TAg）抗体和低水平的BKV病毒外壳蛋白Ag的血凝抑制抗体。通过补体结合在肿瘤中发现BKV TAg。肿瘤DNA的印迹杂交分析显示，在肿瘤细胞中存在游离和整合的BKV基因组。BKF DNA静脉内和皮下接种在免疫抑制或免疫活性的仓鼠中不致癌;当脑内接种时，它是弱致癌的。
Tumors were induced by BK virus (BKV) inoculated i.v. in 3 wk old Syrian golden hamsters immunosuppressed with anti-lymphocyte serum or methylprednisolone acetate alone or in association with .gamma.-radiation (60Co). The induced neoplasms were ependymoma, carcinoma of pancreatic islets, lymphoma, osteosarcoma, undifferentiated sarcoma, kidney and renal pelvis carcinoma, pheochromocytoma and hemangiosarcoma. High levels of insulin and glucagon and altered concentrations of glucose were detected in blood of animals with tumors of pancreatic islets. No antibodies of BKV tumor antigen (TAg) and low levels of hemagglutination-inhibiting antibodies to BKV viral coat protein Ag were detected in hamster sera. BKV TAg was found in tumors by complement fixation. Blot hybridization analysis of tumor DNA showed the presence of both free and integrated BKV genomes in tumor cells. BKF DNA inoculated i.v. and s.c. in immunosuppressed or immunocompetent hamsters was not oncogenic; it was weakly oncogenic when inoculated intracerebrally.