SCREENING OF HALOPHILIC BACTERIA AND ALTEROMONAS SPECIES FOR ORGANOPHOSPHORUS HYDROLYZING ENZYME-ACTIVITY
SCREENING OF HALOPHILIC BACTERIA AND ALTEROMONAS SPECIES FOR ORGANOPHOSPHORUS HYDROLYZING ENZYME-ACTIVITY
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DOI：
10.1016/0009-2797(93)90035-w
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发表时间：
1993-06-01
影响因子：
5.1
通讯作者：
SZAFRANIEC, LL
中科院分区：
文献类型：
--
作者：
DEFRANK, JJ;BEAUDRY, WT;SZAFRANIEC, LL
此前，在一种命名为JD6.5的嗜盐细菌分离株中发现了一种G型神经毒剂，可降解酶活性。该微生物初步鉴定为交替单胞菌属的未知种。为了确定这种类型的酶活性是否在交替单胞菌属的其他物种中常见，启动了筛选程序。一些交替单胞菌属物种和5嗜盐细菌菌株进行了培养，并筛选其粗细胞提取物对几种有机磷化学试剂和其他相关化合物的水解活性。还对样品进行了筛选，以确定其与针对来自JD 6.5的纯化酶的单克隆抗体的交叉反应性，以及与基于其N-末端氨基酸序列的DNA探针的杂交。观察到广泛的活性和反应性，表明这些细菌物种中存在的功能相似的酶之间存在显著的异质性。本文所述类型的酶在化学战剂的去污和非军事化方面具有相当大的潜力。
Previously, a G-type nerve agent degrading enzyme activity was found in a halophilic bacterial isolate designated JD6.5. This organism was tentatively identified as an unknown species of the genus Alteromonas. In order to determine whether this type of enzyme activity was common in other species of Alteromonas, a screening program was initiated. A number of Alteromonas species and five halophilic bacterial isolates were cultured and their crude cell extracts screened for hydrolytic activity against several organophosphorus chemical agents and other related compounds. The samples were also screened for cross-reactivity with a monoclonal antibody raised against the purified enzyme from JD6.5 and for hybridization with a DNA probe based on its N-terminal amino acid sequence A wide spectrum of activities and reactivities were seen, suggesting a significant heterogeneity between the functionally similar enzymes that are present in these bacterial species. Enzymes of the type described here have considerable potential for the decontamination and demilitarization of chemical warfare agents.