Acute respiratory distress syndrome induced by a swine 2009 H1N1 variant in mice.
Acute respiratory distress syndrome induced by a swine 2009 H1N1 variant in mice.
复制标题
猪 2009 H1N1 变种在小鼠中引起的急性呼吸窘迫综合征
DOI：
10.1371/journal.pone.0029347
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Liu J
中科院分区：
文献类型：
--
作者：
Zhang Y;Sun H;Fan L;Ma Y;Sun Y;Pu J;Yang J;Qiao J;Ma G;Liu J
由2009 H1N1流感病毒引起的急性呼吸窘迫综合征（ARDS）已被广泛报道，并被认为是2009 H1N1感染重症患者的主要原因。然而，目前尚未开发出针对 2009 H1N1 病毒感染引起的 ARDS 的动物模型。在这里，我们提出了 2009 H1N1 病毒诱导的 ARDS 小鼠模型。小鼠接种了 A/swine/Shandong/731/2009 (SD/09)，这是一种 2009 年 H1N1 流感病毒变异体，血凝素中存在 G222D 突变。每天记录临床症状。通过肺含水量和组织病理学观察评估肺损伤。在接种后的不同时间测量动脉血气、支气管肺泡灌洗液和血液中的白细胞计数、病毒滴度和肺部细胞因子水平。感染SD/09病毒的小鼠表现出典型的ARDS症状，其特征是接种后8-10天致死率达到60%、肺部高度水肿、炎性细胞浸润、肺泡和间质水肿、肺出血、进行性和严重的低氧血症以及促炎细胞因子和趋化因子水平升高。这些结果表明，我们成功建立了2009 H1N1强毒株诱导的ARDS小鼠模型，且无需预先适应，这可能有助于评估2009 H1N1病毒引起的人类ARDS的发病机制或治疗。
Acute respiratory distress syndrome (ARDS) induced by pandemic 2009 H1N1 influenza virus has been widely reported and was considered the main cause of death in critically ill patients with 2009 H1N1 infection. However, no animal model has been developed for ARDS caused by infection with 2009 H1N1 virus. Here, we present a mouse model of ARDS induced by 2009 H1N1 virus. Mice were inoculated with A/swine/Shandong/731/2009 (SD/09), which was a 2009 H1N1 influenza variant with a G222D mutation in the hemagglutinin. Clinical symptoms were recorded every day. Lung injury was assessed by lung water content and histopathological observation. Arterial blood gas, leukocyte count in the bronchial alveolar lavage fluid and blood, virus titers, and cytokine levels in the lung were measured at various times post-inoculation. Mice infected with SD/09 virus showed typical ARDS symptoms characterized by 60% lethality on days 8–10 post-inoculation, highly edematous lungs, inflammatory cellular infiltration, alveolar and interstitial edema, lung hemorrhage, progressive and severe hypoxemia, and elevated levels of proinflammatory cytokines and chemokines. These results suggested that we successfully established an ARDS mouse model induced by a virulent 2009 H1N1 variant without previous adaptation, which may be of benefit for evaluating the pathogenesis or therapy of human ARDS caused by 2009 H1N1 virus.
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影响因子：
6
作者：
Majeski, EI;Paintlia, MK;London, L
通讯作者：
London, L
影响因子：
64.8
作者：
Kash, John C.;Tumpey, Terrence M.;Katze, Michael G.
通讯作者：
Katze, Michael G.
DOI：
10.1164/rccm.200302-305oc
发表时间：
2005-01-15
影响因子：
24.7
作者：
Lang, JD;Figueroa, M;Freeman, BA
通讯作者：
Freeman, BA
DOI：
10.1164/ajrccm.160.6.9901117
发表时间：
1999-12-01
影响因子：
24.7
作者：
Greene, KE;Wright, JR;Martin, TR
通讯作者：
Martin, TR
DOI：
10.1186/cc8208
发表时间：
2009
期刊：
Critical care (London, England)
影响因子：
--
作者：
Bermejo-Martin JF;Ortiz de Lejarazu R;Pumarola T;Rello J;Almansa R;Ramírez P;Martin-Loeches I;Varillas D;Gallegos MC;Serón C;Micheloud D;Gomez JM;Tenorio-Abreu A;Ramos MJ;Molina ML;Huidobro S;Sanchez E;Gordón M;Fernández V;Del Castillo A;Marcos MA;Villanueva B;López CJ;Rodríguez-Domínguez M;Galan JC;Cantón R;Lietor A;Rojo S;Eiros JM;Hinojosa C;Gonzalez I;Torner N;Banner D;Leon A;Cuesta P;Rowe T;Kelvin DJ
通讯作者：
Kelvin DJ