Improving phosphorus acquisition of white clover (Trifolium repens L.) by transgenic expression of plant-derived phytase and acid phosphatase genes
Improving phosphorus acquisition of white clover (Trifolium repens L.) by transgenic expression of plant-derived phytase and acid phosphatase genes
复制标题
DOI：
10.1016/j.plantsci.2009.01.001
复制
发表时间：
2009-04-01
期刊：
影响因子：
5.2
通讯作者：
Wang, Zeng-Yu
中科院分区：
文献类型：
--
作者：
Ma, Xue-Feng;Wright, Elane;Wang, Zeng-Yu
在许多生态系统中，磷是限制作物生产的最缺乏的常量营养素之一。从模式豆科牧草紫花苜蓿中分离到一个植酸酶基因(MtPHY1)和一个紫色酸性磷酸酶基因(MtPAP1)，通过农杆菌介导法将它们导入白三叶(Trifoliumreens L)中，其启动子分别由CaMV35S启动子和根特异性启动子驱动。在转基因植株的根中检测到转录本。转基因植株根质外体中的植酸酶或酸性磷酸酶(APase)活性是野生型对照的3倍以上。在以有机磷(Po)为唯一磷源的砂盆中生长80d后，表现最好的转基因植株的地上部组织干重几乎是对照的两倍，与供应无机磷(PI)的植株相当。在水培(40d)和砂盆(80d)条件下，Po处理转基因植株的相对生物量分别是PI处理的90%和80%以上。相比之下，在水培或砂盆中，PO处理下野生型对照的生物量仅为PI处理的50%左右。此外，转基因植株的地上部P含量显著高于对照。在Po供应的砂盆中，转基因植株积累了比对照高得多的总磷(生长80d后最高可达2.6倍)。结果表明，MtPHY1或MtPAP1基因在白三叶植株中的表达提高了白三叶植株对有机磷的利用能力。(C)2009爱思唯尔爱尔兰有限公司。保留所有权利。
Phosphate is one of the least available macronutrients restricting crop production in many ecosystems. A phytase gene (MtPHY1) and a purple acid phosphatase gene (MtPAP1), both isolated from the model legume Medicago truncatula, were introduced into white clover (Trifolium repens L) by Agrobacterium-mediated transformation, The transgenes were driven by the constitutive CaMV35S promoter or the root-specific MtPT1 promoter. Transcripts were detected in roots of the transgenic plants. Phytase or acid phosphatase (APase) activities in root apoplasts of the transgenic plants were increased up to threefold compared to the wild type control. After the plants were grown 80 days in sand pots supplied with organic phosphorus (Po) as the sole P source, dry weights of shoot tissues of the best performing transgenic plants almost doubled that of the control and were comparable to the counterparts supplied with inorganic phosphorus (Pi). Relative biomass production of the transgenics under Po treatment was over 90% and 80% of that from the Pi treatment when the plants were grown in hydroponics (40 days) and sand pots (80 days), respectively. In contrast, biomass of the wild type controls under Po treatment was only about 50% of the Pi treatment in either hydroponic cultures or sand pots. In addition, shoot P concentrations of the transgenic plants were significantly increased compared to the control. Transgenic plants accumulated much higher amounts of total P (up to 2.6-fold after 80 days of growth) than the control in Po supplied sand pots. The results showed that transgenic expression of MtPHY1 or MtPAP1 in white clover plants increased their abilities of utilizing organic phosphorus in response to P deficiency. (C) 2009 Elsevier Ireland Ltd. All rights reserved.