SCHISTOSOMA-MANSONI - LECTIN-DEPENDENT CYTO-TOXICITY OF HEMOCYTES FROM SUSCEPTIBLE HOST SNAILS, BIOMPHALARIA-GLABRATA
SCHISTOSOMA-MANSONI - LECTIN-DEPENDENT CYTO-TOXICITY OF HEMOCYTES FROM SUSCEPTIBLE HOST SNAILS, BIOMPHALARIA-GLABRATA
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DOI：
10.1016/s0014-4894(85)80030-8
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发表时间：
1985-01-01
影响因子：
2.1
通讯作者：
BAYNE, CJ
中科院分区：
文献类型：
--
作者：
BOSWELL, CA;BAYNE, CJ
正常情况下，良性血细胞来自蜗牛品系（M系）B。glabrata，对S. mansoni，如果首先用凝集素伴刀豆球蛋白A（ConA）处理寄生虫，则对孢子囊阶段具有细胞毒性。ConA结合可与α-甲基甘露糖苷和杀伤是剂量依赖性的。Con-A诱导的细胞毒性的最大水平与来自抗性蜗牛株（13-16-R1）的血细胞遇到未处理的孢子囊时观察到的水平相当。如果单独用凝集素预处理血细胞，则不会诱导细胞毒性反应。一种独特的方法，结合紫外显微镜和重要的荧光染料，曙红Y，用于区分活的和死的孢子囊。该模型可能有助于理解无脊椎动物效应细胞识别和杀死入侵生物的机制。
Normally benign hemocytes from a strain (M-line) of the snail, B. glabrata, susceptible to S. mansoni, became cytotoxic towards the sporocyst stage if the parasite was 1st treated with the lectin, concanavalin A (ConA). ConA binding was inhibitable with .alpha.-methyl mannoside and killing was dose-dependent. Maximal levels of Con-A-induced cytotoxicity were comparable with levels observed when hemocytes from a resistant snail strain (13-16-R1) encountered untreated sporocysts. Induction of the cytotoxic response did not occur if hemocytes alone were pretreated with the lectin. A unique method incorporating UV microscopy and the vital fluorescent dye, eosin Y, was used for discriminating between live and dead sporocysts. This model may prove useful in understanding mechanisms used by invertebrate effector cells in recognition and killing of invading organisms.