Hyaluronan on the surface of tumor cells is correlated with metastatic behavior.
Hyaluronan on the surface of tumor cells is correlated with metastatic behavior.
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DOI：
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发表时间：
1995-01
期刊：
影响因子：
11.2
通讯作者：
Lurong Zhang;C. Underhill;Lieping Chen
中科院分区：
文献类型：
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作者：
Lurong Zhang;C. Underhill;Lieping Chen
在本研究中，我们检查了表达不同水平的细胞表面透明质酸的肿瘤细胞的转移潜力。我们使用流式细胞术分离了 B16-F1 小鼠黑色素瘤细胞系的亚群，这些细胞系在其表面表达高量 (HA-H) 或低量 (HA-L) 透明质酸。由于其合成速率，这两个细胞亚群的细胞表面透明质酸含量存在 32 倍的差异。然而，这些细胞系在体外生长速率、对自然杀伤介导的细胞毒性的敏感性或细胞表面蛋白 CD44、ICAM-1 和 GMP-140 的表达方面彼此没有差异。当这些细胞被皮下注射时，它们都形成了皮下细胞。肿瘤大小大致相同。然而，当注射到小鼠尾静脉时，HA-H细胞在肺部形成更多数量的结节，并且比HA-L细胞导致更快的死亡率。透明质酸的存在确实增强了 HA-H 细胞与培养的表达 CD44 的内皮细胞的相互作用。因此，透明质酸和 CD44 之间相互作用的增强可能促进了肿瘤栓塞的形成，进而增加了肿瘤细胞被困在肺部的机会。综上所述，这些结果表明透明质酸可能在肿瘤转移过程中发挥关键作用。
In the present study, we examined the metastatic potential of tumor cells expressing different levels of cell surface hyaluronan. We used flow cytometry to isolate subsets of the B16-F1 mouse melanoma cell line that expressed either high (HA-H) or low (HA-L) amounts of hyaluronan on their surfaces. These two subsets of cells showed a 32-fold difference in the amount of cell surface hyaluronan, due to its rate of synthesis. However, these cell lines did not differ from each other with regard to their in vitro growth rates, susceptibility to natural killer-mediated cytotoxicity, or the expression of the cell surface proteins CD44, ICAM-1, and GMP-140. When these cells were injected s.c., they both formed s.c. tumors of approximately the same size. However, when injected into the tail vein of mice, the HA-H cells formed a greater number of nodules in the lungs and caused a faster rate of mortality than the HA-L cells. The presence of hyaluronan did enhance the interaction of the HA-H cells with cultured endothelial cells that expressed CD44. Thus, it is possible that enhanced interactions between hyaluronan and CD44 promoted the formation of tumor embolisms which, in turn, increased the chances that the tumor cells would be trapped in the lungs. Taken together, these results suggest that hyaluronan may play a critical role in the process of tumor metastasis.