Surface-Carboxylated Nanocellulose-Based Bioadaptive Scaffolds for Cell Culture
Surface-Carboxylated Nanocellulose-Based Bioadaptive Scaffolds for Cell Culture
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DOI：
10.1007/s10570-021-04154-5
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发表时间：
2021-08-25
期刊：
影响因子：
5.7
通讯作者：
Kitaoka, Takuya
中科院分区：
文献类型：
--
作者：
Hatakeyama, Mayumi;Kitaoka, Takuya
细胞外基质（ECM）对细胞附着、增殖和分化至关重要;因此，开发生物适应性ECM模拟细胞培养支架是组织工程研究的活跃领域。胶原蛋白（刚性的胶原形态）和透明质酸（羧基以规则的方式显示）是体内发现的典型ECM组分。在这项工作中，我们使用木材衍生的结晶纤维素纳米纤维（CNFs）为细胞培养提供生物适应性微环境。使用2，2，6，6-四甲基哌啶1-氧基（克里思）催化氧化木材CNFs得到具有不同羧基含量的结晶纳米纤维支架，TEMPO氧化的CNFs（TOCNFs）的范围为0.31至1.60 mmol g（-1）。将小鼠成纤维细胞置于亲水性TOCNF包被的基底上，该基底是透明的，并显示出天然纤维素I晶体结构;观察到羧基含量为0.8-1.0 mmol g（-1）的TOCNF具有良好的细胞增殖，尽管完整的CNF是生物惰性的，过量的羧酸盐会对细胞生长产生负面影响。无论是具有纤维素II结构的丝光TOCNFs，还是具有不规则表面羧基的羧甲基化CNFs，都不利于细胞增殖。因此，具有表面羧基的规则排列的无异源的结晶TOCNF的刚性微形态将是提供生物适应性ECM模拟细胞微环境的关键。[图形]。
The extracellular matrix (ECM) is critical to cell attachment, proliferation, and differentiation; therefore, development of bioadaptive ECM-mimetic cell culture scaffolds is an active area of research in tissue engineering. Collagen (rigid nanofiber morphology) and hyaluronan (carboxy groups displayed in a regular manner) are typical ECM components found in vivo. In this work, we used wood-derived crystalline cellulose nanofibers (CNFs) to provide bioadaptive microenvironments for cell culture. Catalytic oxidation of wood CNFs using 2,2,6,6-tetramethylpiperidine 1-oxyl (TEMPO) afforded crystalline nanofibrous scaffolds with various carboxy contents, ranging from 0.31 to 1.60 mmol g(-1), of the TEMPO-oxidized CNFs (TOCNFs). Mouse fibroblast cells were subjected to the hydrophilic TOCNF-coated substrates, which were transparent and exhibited a native cellulose I crystalline structure; good cell proliferation was observed for the TOCNFs with carboxy content of 0.8-1.0 mmol g(-1), although intact CNF was bioinert and an excess of carboxylates negatively impacted cell growth. Neither mercerized TOCNFs with a cellulose II structure nor carboxymethylated CNFs with irregular surface carboxy groups contributed to cell proliferation. Therefore, the rigid nanofiber morphology of xeno-free, crystalline TOCNFs with regular alignment of surface carboxy groups would hold the key to providing bioadaptive, ECM-mimetic cellular microenvironments.[GRAPHICS].