An orderly retreat: Dedifferentiation is a regulated process
An orderly retreat: Dedifferentiation is a regulated process
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DOI：
10.1073/pnas.0306983101
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发表时间：
2004-05-04
影响因子：
11.1
通讯作者：
Shaulsky, G
中科院分区：
文献类型：
--
作者：
Katoh, M;Shaw, C;Shaulsky, G
分化是一个高度调节的过程，细胞在此过程中变得特化以执行特定功能并失去执行其他功能的能力。相反，去分化是一个遗传决定的过程，还是仅仅是分化状态的不受调节的丧失，这个问题还没有得到解决。我们在这里表明，在社会阿米巴Dictyosteelium discoideum的去分化依赖于一系列的事件，是独立的原始发展状态，并涉及一组特定的基因的协调表达。这些基因之一的缺陷，组氨酸激酶dhkA，改变去分化的动力学和解偶联的去分化事件的进展。这些观察建立去分化作为一个遗传决定的过程，并建议存在一个发育检查点，确保返回到未分化状态的路径。
Differentiation is a highly regulated process whereby cells become specialized to perform specific functions and lose the ability to perform others. In contrast, the question of whether dedifferentiation is a genetically determined process, or merely an unregulated loss of the differentiated state, has not been resolved. We show here that dedifferentiation in the social amoeba Dictyostelium discoideum relies on a sequence of events that is independent of the original developmental state and involves the coordinated expression of a specific set of genes. A defect in one of these genes, the histidine kinase dhkA, alters the kinetics of dedifferentiation and uncouples the progression of dedifferentiation events. These observations establish dedifferentiation as a genetically determined process and suggest the existence of a developmental checkpoint that ensures a return path to the undifferentiated state.