Activation of the neural pathway from the dorsolateral bed nucleus of the stria terminalis to the central amygdala induces anxiety-like behaviors
Activation of the neural pathway from the dorsolateral bed nucleus of the stria terminalis to the central amygdala induces anxiety-like behaviors
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DOI：
10.1111/ejn.14165
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发表时间：
2018-11-01
影响因子：
3.4
通讯作者：
Minami, Masabumi
中科院分区：
文献类型：
--
作者：
Yamauchi, Naoki;Takahashi, Daiki;Minami, Masabumi
终纹床核（BNST）和中央杏仁核（CeA）组成一个前脑单位，被称为“延伸杏仁核”。这两个核团相互发送密集的投射，并参与了负面情绪状态的调节，包括焦虑和恐惧。本研究采用光遗传学技术，以检查是否CeA投射背外侧BNST（dlBNST）神经元末梢的刺激会影响焦虑样行为在雄性Sprague-Dawley大鼠。光刺激的CeA投射dlBNST神经元末梢产生焦虑的影响，在高架十字迷宫测试。这一发现与先前的报道不一致，该报道显示投射到外侧下丘脑（LH）和腹侧被盖区（VTA）的BNST神经元的光遗传学刺激产生抗焦虑作用而不是致焦虑作用。为了解决这个问题，进行电生理分析，以表征投射到CeA，LH和VTA的dlBNST神经元。dlBNST神经元可根据其对去极化和超极化电流注入的响应在电生理学上分为三种不同的细胞类型（I-III型）。全细胞膜片钳记录显示，超过60%的CeA投射dlBNST神经元的II型，而约80%的LH和VTA投射dlBNST神经元的III型。这些电生理结果将有助于阐明BNST神经元在焦虑样行为调节过程中的异质性机制。
The bed nucleus of the stria terminalis (BNST) and the central amygdala (CeA) comprise a forebrain unit that has been described as the "extended amygdala". These two nuclei send dense projections to each other and have been implicated in the regulation of negative emotional states, including anxiety and fear. The present study employed an optogenetic technique to examine whether stimulation of CeA-projecting dorsolateral BNST (dlBNST) neuron terminals would influence anxiety-like behaviors in male Sprague-Dawley rats. Photostimulation of CeA-projecting dlBNST neuron terminals produced anxiogenic effects in an elevated plus maze test. This finding is inconsistent with previous reports showing that optogenetic stimulation of BNST neurons projecting to the lateral hypothalamus (LH) and ventral tegmental area (VTA) produces anxiolytic rather than anxiogenic effects. To address this issue, electrophysiological analyses were conducted to characterize dlBNST neurons projecting to the CeA, LH, and VTA. dlBNST neurons can be electrophysiologically classified into three distinct cell types (types I-III) according to their responses to depolarizing and hyperpolarizing current injections. Whole-cell patch-clamp recordings revealed that more than 60% of the CeA-projecting dlBNST neurons were type II, whereas approximately 80% of the LH- and VTA-projecting dlBNST neurons were type III. These electrophysiological results will help elucidate the mechanisms underlying the heterogeneity of BNST neurons during the regulation of anxiety-like behaviors.