H2S mediates increased interleukin (IL)-1β and IL-18 production in leukocytes from patients with periodontitis
H2S mediates increased interleukin (IL)-1β and IL-18 production in leukocytes from patients with periodontitis
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DOI：
10.1080/20002297.2019.1617015
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发表时间：
2019-01-01
影响因子：
4.5
通讯作者：
Dahlen, Gunnar
中科院分区：
文献类型：
--
作者：
Basic, Amina;Serino, Giovanni;Dahlen, Gunnar
Background: The mechanisms involved in the interplay between the bacteria and the host cells in periodontitis are not fully understood. Aim: To investigate the effect of the bacterial metabolite H2S on the pro-inflammatory cytokines interleukin (IL)-1 beta and IL-18 from periodontitis patients and healthy controls, and to evaluate the composition of the subgingival microbiota with its capacity to produce H2S. Methods: Subgingival bacterial samples from patients with periodontitis (N=32) and healthy controls (N=32) were investigated for H2S production and bacterial composition. Peripheral blood mononuclear cells (PBMCs) were cultured in the presence/absence of 1mM H2S for 24h and cytokine concentrations were measured. Results: Subgingival plaque from periodontitis patients had more H2S producing bacteria and produced more H2S, than healthy controls. PBMCs exposed to H2S secreted significantly more IL-1 ss and IL-18 (p
