RELATIVE ABUNDANCE OF ALZHEIMER A-BETA AMYLOID PEPTIDE VARIANTS IN ALZHEIMER-DISEASE AND NORMAL AGING
RELATIVE ABUNDANCE OF ALZHEIMER A-BETA AMYLOID PEPTIDE VARIANTS IN ALZHEIMER-DISEASE AND NORMAL AGING
复制标题
DOI：
10.1073/pnas.91.18.8378
复制
发表时间：
1994-08-30
影响因子：
11.1
通讯作者：
TERENIUS, L
中科院分区：
文献类型：
--
作者：
NASLUND, J;SCHIERHORN, A;TERENIUS, L
阿尔茨海默病Aβ淀粉样多肽(Aβ)是与阿尔茨海默病(AD)相关的淀粉样蛋白的主要蛋白质成分。我们已经确定了10名散发性AD患者、2名携带阿尔茨海默病淀粉样前体蛋白基因特定突变的家族性AD患者和5名非痴呆老年对照组的大脑淀粉样蛋白Aβ结构变体的相对丰度。使用了一种基于Aβ淀粉样蛋白极不溶的分离程序。对纯化的、未消化的Aβ进行N末端测序和电喷雾电离质谱分析。在所有被分析的大脑中检测到三个主要的Aβ变异体--Aβ-(1-40)、Aβ-(1-42)和Aβ-(11-42)。散发性AD的主要变异是Aβ-(1-40)，而非痴呆老年对照组的主要Aβ变异是Aβ-(1-42)。Aβ-(1-40)/A-β-(1-42)比值在非痴呆组和散发性AD组之间相差10倍。
The Alzheimer A beta amyloid peptide (A beta) is the principal proteinaceous component of amyloid associated with Alzheimer disease (AD). We have determined the relative abundance of A beta structural variants present in amyloid from brains of 10 individuals with sporadic AD, 2 individuals with familial AD carrying specific mutations in the Alzheimer amyloid precursor protein gene, and 5 nondemented elderly controls. A procedure of isolation based on the extreme insolubility of A beta amyloid was used. The purified, nondigested A beta was analyzed by N-terminal sequencing and electrosprayionization mass spectrometry. Three principal A beta variants were detected-A beta-(1-40), A beta-(1-42), and A beta-(11-42)-in all brains analyzed. The predominant variant in sporadic AD was A beta-(1-40), whereas the principal A beta variant in nondemented elderly controls was A beta-(1-42). The ratio A beta-(1-40)/A beta-(1-42) differed by 10-fold between brains from nondemented controls and those with sporadic AD.