Project Baseline: An unprecedented resource to study plant evolution across space and time
Project Baseline: An unprecedented resource to study plant evolution across space and time
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DOI：
10.3732/ajb.1500313
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发表时间：
2016-01-01
影响因子：
3
通讯作者：
Weis, Arthur E.
中科院分区：
文献类型：
--
作者：
Etterson, Julie R.;Franks, Steven J.;Weis, Arthur E.
研究前提：项目基线是一个种子库，提供了一个前所未有的机会，在一个快速的环境变化的时代检查微观演变的空间和时间方面。在接下来的50年里，生物学家将从这个时间胶囊的种子中提取过去种群的遗传代表性样本，并与现代样本同时生长，以检测在此期间发生的任何表型和分子进化。方法：我们使用协议精心开发了这个活基因组库，通过收集广泛的多个种群和物种来提高其实验价值。地理范围内的地点，有可能被保存到未来。种子与地点和种群数据一起登记，并在最大限度地延长种子寿命的条件下按母系储存。这个开放获取的资源将定期提供给研究人员，以评估当代evolution.Key结果：迄今为止，项目基线收集包括100-200母系的61个物种，每个收集超过831个人口的网站，有可能被保存到未来在美国（类似于78,000母系）。我们精心设计的收集避免了一些问题，这些问题可能会影响“复活”研究的结果，这些研究涉及自然保存的或偶然可用的现有种子收集。复活的方法可以与长期-在接下来的50年里，我们将使用成熟的和更新的技术来阐明遗传变化，从而极大地提高我们对表型和进化过程的时空变化的理解。隐藏其中
PREMISE OF THE STUDY: Project Baseline is a seed bank that offers an unprecedented opportunity to examine spatial and temporal dimensions of micro-evolution during an era of rapid environmental change. Over the upcoming 50 years, biologists will withdraw genetically representative samples of past populations from this time capsule of seeds and grow them contemporaneously with modern samples to detect any phenotypic and molecular evolution that has occurred during the intervening time.METHODS: We carefully developed this living genome bank using protocols to enhance its experimental value by collecting from multiple populations and species across a broad geographical range in sites that are likely to be preserved into the future. Seeds are accessioned with site and population data and are stored by maternal line under conditions that maximize seed longevity. This open-access resource will be available to researchers at regular intervals to evaluate contemporary evolution.KEY RESULTS: To date, the Project Baseline collection includes 100-200 maternal lines of each of 61 species collected from over 831 populations on sites that are likely to be preserved into the future across the United States (similar to 78,000 maternal lines). Our strategically designed collection circumvents some problems that can cloud the results of "resurrection" studies involving naturally preserved or existing seed collections that are available fortuitously.CONCLUSIONS: The resurrection approach can be coupled with long-established and newer techniques over the next five decades to elucidate genetic change and thereby vastly improve our understanding of temporal and spatial changes in phenotype and the evolutionary processes underlying it.