Coexpression of myosin isoforms in muscle of patients with neurogenic disease
Coexpression of myosin isoforms in muscle of patients with neurogenic disease
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神经源性疾病患者肌肉中肌球蛋白亚型的共表达
DOI：
10.1002/mus.880120809
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发表时间：
1989
期刊：
影响因子：
3.4
通讯作者：
S. Shafiq
中科院分区：
文献类型：
--
作者：
J. A. Sawchak;Simeon Lewis;S. Shafiq
使用三种充分表征的抗肌球蛋白重链单克隆抗体（McAb）作为免疫细胞化学试剂，研究肌球蛋白亚型表达与人肌肉中腺苷三磷酸酶（ATP酶）定义的纤维类型的关系。活检标本来自神经源性肌肉疾病患者，其肌肉表现出纤维类型分组和分组萎缩。单克隆抗体的使用揭示了迄今未被识别的多种肌球蛋白亚型的共表达在选定的纤维在病理样品中，这是不明显的ATP酶反应，并不存在于正常肌肉。含有多种肌球蛋白亚型的纤维可能正在经历神经定向纤维类型转化。此外，在神经源性标本中的一小部分纤维表达了一种“产前”肌球蛋白，表明存在再生纤维。我们还证明了免疫细胞化学证据的持久性成人慢肌球蛋白在失神经支配的成熟人骨骼肌，尽管神经支配的必要性，维持这种肌球蛋白亚型的表达提供了其他。
Three well‐characterized antimyosin heavy chain monoclonal antibodies (McAbs) were used as immunocytochemical reagents to study myosin iso‐form expression in relationship to adenosine triphosphatase (ATPase) defined fiber types in human muscle. The biopsy specimens were from patients with neurogenic muscle disease whose muscle exhibited fiber type grouping and group atrophy. The use of McAbs revealed heretofore unrecognized coexpression of multiple myosin isoforms in selected fibers in the pathologic samples which was not apparent with ATPase reactions and not present in normal muscle. The fibers containing multiple myosin isoforms were probably undergoing neurally directed fiber type transformation. Furthermore, a small population of fibers in neurogenic specimens expressed a “prenatal” myosin signifying the presence of regenerating fibers. We also demonstrated immunocytochemical evidence of the persistence of adult slow myosin in denervated mature human skeletal muscle despite the reputed necessity of innervation for maintenance of expression of this myosin isoform proffered by others.