A rapid and easy method forthe qualitative detection of intracellular deposition of inhaled nanoparticles
A rapid and easy method forthe qualitative detection of intracellular deposition of inhaled nanoparticles
复制标题
一种快速简便的定性检测吸入纳米颗粒细胞内沉积的方法
DOI：
10.1016/j.nano.2011.02.004
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发表时间：
2011
期刊：
影响因子：
5.5
通讯作者：
Todo T
中科院分区：
文献类型：
--
作者：
Nakajima H;Ozaki K;Hongyo T;Narama I;Todo T
近年来，纳米颗粒暴露风险越来越受到研究界和公众的关注。然而，纳米颗粒的分析受到其小尺寸的阻碍，这阻碍了在细胞和组织中检测纳米颗粒的方法的发展。对于纳米颗粒暴露的风险评估，重要的是测量肺组织中沉积材料的确切量。使用纳米颗粒暴露装置，A/JJmsSlc小鼠经气管长期暴露于锐钛矿型二氧化钛颗粒。使用显微镜集成激光拉曼光谱仪检测从暴露于颗粒的小鼠获得的肺洗液中的差异染色的巨噬细胞。这种检测方法允许快速和容易的样品收集和定性分析，该方法可能是有用的，在暴露于严重污染的环境中的工人进行大规模的评估与纳米粒子。来自临床编辑：本文讨论了一种显微镜集成激光拉曼光谱仪的方法来测量沉积在肺组织中的纳米颗粒的确切数量。这种方法可以快速样品收集，定性分析，并可能是有用的大规模评价。
In recent years, nanoparticle exposure risk has drawn increasing attention from the research community and the general public. However, analysis of nanoparticles is hindered by their small size, which prevents the development of methods for their detection in cells and tissues. For risk assessment of nanoparticle exposure, it is important to measure the exact amount of deposited material in pulmonary tissue. Using a nanoparticle exposure device, A/JJmsSlc mice were chronically exposed transtracheally to anatase-type titanium dioxide particles. A microscope-integrated laser Raman spectrometer was used to detect differentially stained macrophages in a pulmonary wash obtained from the mice exposed to the particles. This detection method allowed rapid and easy sample collection and qualitative analysis, and the method may be useful for conducting large-scale evaluations in workers exposed to environments heavily contaminated with nanoparticles. FROM THE CLINICAL EDITOR: This paper discusses a microscope-integrated laser Raman spectrometer method to measure the exact amount of nanoparticles deposited in pulmonary tissue. This method allows rapid sample collection, qualitative analysis, and may be useful for large-scale evaluations.