Gastric and duodenal mucosal ornithine decarboxylase and damage after corticosterone.
Gastric and duodenal mucosal ornithine decarboxylase and damage after corticosterone.
复制标题
胃和十二指肠粘膜鸟氨酸脱羧酶和皮质酮后的损伤。
DOI：
10.1152/ajpgi.1990.258.6.g942
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Johnson,LR
中科院分区：
文献类型：
--
作者：
Wang,JY;Johnson,LR
我们最近发现，压力增加胃和十二指肠鸟氨酸脱羧酶（ODC）的活性和损害这两个组织。目前的研究调查是否皮质酮诱导ODC在大鼠胃和十二指肠粘膜的活动，并比较血浆皮质酮水平应激和皮质酮治疗后，以确定是否这种激素介导的应激对粘膜的影响。大鼠禁食22 h，置于束缚笼中，浸入水中至剑突6 h。应激使血浆皮质酮水平显著升高，是对照组的10.5倍，并持续应激时间。在单次注射皮质酮（5 mg/kg sc）后1 h观察到最大增加，为对照值的11.1倍。到6小时，血浆皮质酮已恢复到正常水平。单次注射皮质酮对胃粘膜无影响，但十二指肠ODC在注射后4 ~ 8h显著增加，6 h达高峰。组织学检查显示两种组织均无损伤。连续3天每天3次给予皮质酮显著提高了ODC活性，并产生了显著的显微损伤。胃的表面上皮被破坏，许多表面细胞脱落，十二指肠粘膜中大多数绒毛缺失。皮质酮还显著降低两种组织的DNA和RNA含量。用DL-α-二氟甲基鸟氨酸抑制ODC还降低了DNA、RNA和蛋白质含量，加重了损伤。（250字处删节）
We recently found that stress increases gastric and duodenal ornithine decarboxylase (ODC) activity and damages both tissues. The current study investigated whether corticosterone induces ODC activity in gastric and duodenal mucosa in rats and compared plasma corticosterone levels after stress and treatment with corticosterone to determine whether this hormone mediated the effects of stress on the mucosa. Rats were fasted 22 h, placed in a restraint cage, and immersed in water to the xiphoid process for 6 h. Stress markedly increased plasma corticosterone levels; the increase was 10.5 times control and lasted the duration of stress. The maximum increase was observed 1 h after a single injection of corticosterone (5 mg/kg sc) and represented 11.1 times control values. By 6 h, plasma corticosterone had returned to normal levels. A single injection of corticosterone had no effect on the gastric mucosa, but duodenal ODC was increased significantly from 4 to 8 h after injection, peaking at 6 h. Histological examination revealed no damage in either tissue. Administration of corticosterone three times daily for 3 days dramatically elevated ODC activity and produced significant microscopic damage. The surface epithelium of the stomach was disrupted, with many surface cells shed, and most villi were absent from the duodenal mucosa. Corticosterone also markedly decreased DNA and RNA content of both tissues. Inhibition of ODC with DL-alpha-difluoromethylornithine additionally decreased DNA, RNA, and protein content, exacerbating the damage.(ABSTRACT TRUNCATED AT 250 WORDS)