The human C-type lectin CLECSF8 is a novel monocyte/macrophage endocytic receptor
The human C-type lectin CLECSF8 is a novel monocyte/macrophage endocytic receptor
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DOI：
10.1002/eji.200324230
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发表时间：
2004-01-01
影响因子：
5.4
通讯作者：
Fernández-Ruiz, E
中科院分区：
文献类型：
--
作者：
Arce, I;Martínez-Muñoz, L;Fernández-Ruiz, E
细胞表面凝集素受体在巨噬细胞的功能中起重要作用。在本文中，我们已经鉴定和表征了小鼠Mcl/Clecsf 8的人直系同源物。人CLECSF 8编码属于人钙依赖性凝集素家族（C型凝集素）的215个氨基酸的II型膜糖蛋白。CLECSF 8的胞质尾区缺乏共有信号传导基序，并且其胞外区显示单个碳水化合物识别结构域（CRD）。CLECSF 8基因定位于染色体12 p13上靠近MINCLE的NK基因复合体的端粒区。CLECSF 8 mRNA显示单核细胞/巨噬细胞表达模式。CLECSF 8对瞬时转染细胞的生化分析显示30 kDa的糖蛋白。受体的交联导致快速内化，表明CLECSF 8构成内吞受体。
Cell surface lectin receptors play important roles in the function of macrophages. Herein, we have identified and characterized the human orthologue of the mouse Mcl/Clecsf8. Human CLECSF8 codes for a type II membrane glycoprotein of 215 amino acids that belongs to the human calcium-dependent lectin family (C-type lectin). The cytoplasmic tail of CLECSF8 lacks consensus signaling motifs and its extracellular region shows a single carbohydrate recognition domain (CRD). The CLECSF8 gene has been localized on the telomeric region of the NK gene complex on chromosome 12p13 close to MINCLE. CLECSF8 mRNA shows a monocyte/macrophage expression pattern. Biochemical analysis of CLECSF8 on transiently transfected cells showed a glycoprotein of 30 kDa. Cross-linking of the receptor leads to a rapid internalization suggesting that CLECSF8 constitutes and endocytic receptor.