SUCCESSFUL ARTIFICIAL-INSEMINATION IN THE GIANT PANDA (AILUROPODA-MELANOLEUCA) MELANOLEUCA) AT UENO ZOO
SUCCESSFUL ARTIFICIAL-INSEMINATION IN THE GIANT PANDA (AILUROPODA-MELANOLEUCA) MELANOLEUCA) AT UENO ZOO
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DOI：
10.1002/zoo.1430080104
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发表时间：
1989-01-01
期刊：
影响因子：
1.3
通讯作者：
SAITO, K
中科院分区：
文献类型：
--
作者：
MASUI, M;HIRAMATSU, H;SAITO, K
上野动物园连续两年(1885年和1986年)通过人工授精成功繁殖大熊猫(Ailuropoda Blackoluca)。第一只幼崽出生于1985年6月，因被熊猫妈妈碾压，不幸在出生43小时后死亡；第二只幼崽出生于1986年6月，一直健康成长。用安定(0.1 mg/kg)和盐酸氯胺酮(4.0~5.0 mg/kg)固定后进行电射精和人工授精。用直径2.0 cm的直肠探头，以八环为电极，采用电射精法采集雄性大熊猫精液。用3V(30-40 mA)刺激雄性，间隔5秒，持续5秒。雌性大熊猫在1985年2月下旬至3月上旬以及1986年1月中旬至2月上旬期间发情。行为发情高峰期尿总雌激素排泄量增加，妊娠二醇水平逐渐升高，人工授精。第一次妊娠的妊娠长度为110天，第二次妊娠的妊娠长度为121天。
Successful breeding of the giant panda (Ailuropoda melanoleuca) following artificial insemination was achieved at the Ueno Zoo in 2 consecutive years (1885 and 1986). The first cub, born in June 1985, unfortunately died 43 hours after birth from being crushed by the mother panda; the second cub, born in June 1986, has been growing in good health. Electroejaculation and artificial insemination procedures were performed after immobilization with diazepam (0.1 mg/kg) and ketamine HCL (4.0-5.0 mg/kg). Semen of the male panda was collected by electroejaculation using a rectal probe with a diameter of 2.0 cm and with eight rings as electrodes. Stimulation of the male was given with 3 V (30-40 mA) over a 5-sec period with 5-sec intervals. The female panda exhibited estrus between late February and early March in 1985 and also between mid-January and early February 1986. Increased excretion of urinary total estrogen showed coincidentally at maximum behavioral estrus, and a gradual rise of pregnanediol level was followed by artificial insemination. The gestational length for the first pregnancy was 110 days and that of the second 121 days.