High-Throughput Screening Assays to Identify Plant Natural Products with Antifungal Properties Against Fusarium oxysporum.
High-Throughput Screening Assays to Identify Plant Natural Products with Antifungal Properties Against Fusarium oxysporum.
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DOI：
10.1007/978-1-0716-1795-3_14
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发表时间：
2022
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尖孢镰刀菌（Fusarium oxysporum）是一种跨界真菌病原体，不仅能引起毁灭性的植物维管疾病，而且还能机会性地感染人类。在这里，我们描述了两个高通量筛选试验，刃天青细胞活力测定和光密度测定，筛选培养的植物细胞的天然产物与抗真菌特性对F.尖孢菌属用茉莉酸甲酯或与F.尖孢菌，分别作为非生物激发子和生物激发子，我们鉴定了三种产生抑制真菌生长的物质的细胞系。我们的程序验证了强大的潜力相结合的高通量方法发现新的抗病原体的线索。
Fusarium oxysporum is a cross-kingdom fungal pathogen that not only causes devastating plant vascular diseases but can also opportunistically infect humans. Here we describe two high-throughput screening assays, a resazurin cell viability assay and an optical density assay, to screen natural products from cultured plant cells with antifungal properties against a clinical isolate of F. oxysporum. After elicitation by applying methyl jasmonate or by co-culture with F. oxysporum, as an abiotic elicitor and a biotic elicitor, respectively, we identified three cell lines that produce materials that inhibit fungal growth. Our procedure validates the powerful potential of combining high-throughput methods for the discovery of novel anti-pathogenic leads.