Flamingo, a seven-pass transmembrane cadherin, cooperates with Netrin/Frazzled in Drosophila midline guidance.
Flamingo, a seven-pass transmembrane cadherin, cooperates with Netrin/Frazzled in Drosophila midline guidance.
复制标题
Flamingo 是一种七次跨膜钙粘蛋白，与 Netrin/Frazzled 合作进行果蝇中线引导。
DOI：
10.1111/gtc.12202
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发表时间：
2015
期刊：
影响因子：
2.1
通讯作者：
Suzuki T*
中科院分区：
文献类型：
--
作者：
Organisti C;Hein I;Grunwald Kadow IC*;Suzuki T*
在中枢神经系统发育过程中，需要多种引导信号和受体以及细胞粘附分子来引导轴突穿过中线并沿着前后轴。在果蝇中，连合轴突通过 Frazzled (Fra) 受体感知中线引诱剂 Netrin A 和 B (Net)。尽管它们很重要，但 Netorfra 的缺乏仅影响某些连合，这表明其他分子可以实现此功能。最近，平面细胞极性（PCP）蛋白与脊椎动物和无脊椎动物系统的中线轴突引导有关。在此，我们报道非典型钙粘蛋白和 PCP 分子 Flamingo/Starry night (Fmi/Stan) 在中线发育过程中与 Net/Fra 信号传导共同作用。额外的去除会大大增加网络/framutants中的引导缺陷。救援和结构域删除实验表明，Fmi 信号传导可能通过以部分同质的方式介导轴突束颤来促进连合寻路。总而言之，我们的结果表明，在穿过中线的轴突寻路过程中，除了 Net/Fra 引导系统之外，通过 Fmi 进行的接触介导的细胞粘附也发挥作用，强调了 PCP 分子在脊椎动物和无脊椎动物中线发育过程中的重要性。
During central nervous system development, several guidance cues and receptors, as well as cell adhesion molecules, are required for guiding axons across the midline and along the anterior–posterior axis. InDrosophila, commissural axons sense the midline attractants Netrin A and B (Net) through Frazzled (Fra) receptors. Despite their importance, lack ofNetorfraaffects only some commissures, suggesting that additional molecules can fulfill this function. Recently, planar cell polarity (PCP) proteins have been implicated in midline axon guidance in both vertebrate and invertebrate systems. Here, we report that the atypical cadherin and PCP molecule Flamingo/Starry night (Fmi/Stan) acts jointly with Net/Fra signaling during midline development. Additional removal offmistrongly increases the guidance defects inNet/framutants. Rescue and domain deletion experiments suggest that Fmi signaling facilitates commissural pathfinding potentially by mediating axonal fasciculation in a partly homophilic manner. Altogether, our results indicate that contact‐mediated cell adhesion via Fmi acts in addition to the Net/Fra guidance system during axon pathfinding across the midline, underlining the importance of PCP molecules during vertebrates and invertebrates midline development.