Photopic Negative Response Obtained Using a Handheld Electroretinogram Device: Determining the Optimal Measure and Repeatability.
Photopic Negative Response Obtained Using a Handheld Electroretinogram Device: Determining the Optimal Measure and Repeatability.
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DOI：
10.1167/tvst.5.4.8
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发表时间：
2016-07
影响因子：
3
通讯作者：
Crowston JG
中科院分区：
文献类型：
--
作者：
Wu Z;Hadoux X;Hui F;Sarossy MG;Crowston JG
目的确定全视野视网膜电图（ERG）明视负反应（PhNR）的最佳重复性水平，并使用手持式非散瞳ERG系统和自粘皮肤电极检查其在不同条件下的重复性。在20名正常参与者的双眼中进行多次ERG记录（每次使用200次扫描），以比较不同PhNR测量和不同测试条件下（检查者之间和检查者之间以及会话之间）的重复性系数（CoR;其中95%的重测差异预计位于）。当包括三个记录时，PhNR谷与b波峰和b波峰与a波谷振幅之间的比值（PhNR/B比值）显示出相对于其有效动态范围（30 ± 4%）的最低CoR。在任一阶段的7次测量（4只右眼和3只左眼）中，PhNR/B比值均无显著变化（P ≥ 0.100），不同测试条件之间的CoR也无显著差异（P = 0.314）。PhNR/B比率是最小化变异性的量度，并且使用具有自粘皮肤电极的新型手持式ERG系统和本研究中描述的方案在不同测试条件下和多次记录下进行的测量是相当的。PhNR可以使用一种易于实施的技术进行测量，用于临床和研究目的，该技术在访视内和访视之间以及检查者之间保持一致。
To determine the measure of the photopic negative response (PhNR) of the full-field electroretinogram (ERG) that exhibits the optimal level of test-retest repeatability, and examine its repeatability under different conditions using a handheld, nonmydriatic ERG system and self-adhering skin electrodes. Multiple ERG recordings (using 200 sweeps each) were performed in both eyes of 20 normal participants at two different sessions to compare its coefficient of repeatability (CoR; where 95% of the test-retest difference is expected to lie) between different PhNR measures and under different testing conditions (within and between examiners, and between sessions). The ratio between the PhNR trough to b-wave peak and b-wave peak to a-wave trough amplitude (PhNR/B ratio) exhibited the lowest CoR relative to its effective dynamic range (30 ± 4%) when including three recordings. There were no significant changes in the PhNR/B ratio over seven measurements (4 right and 3 left eyes) at either session (P ≥ 0.100), or significant difference in its CoR between different testing conditions (P = 0.314). The PhNR/B ratio was the measure that minimized variability, and its measurements using a novel handheld ERG system with self-adhering skin electrodes and the protocols described in this study were comparable under different testing conditions and over multiple recordings. The PhNR can be measured for clinical and research purposes using a simple-to-implement technique that is consistent within and between visits, and also between examiners.