PNRC is a unique nuclear receptor coactivator that stimulates RNA polymerase III-dependent transcription.
PNRC is a unique nuclear receptor coactivator that stimulates RNA polymerase III-dependent transcription.
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PNRC是一种独特的核受体共激活因子，可刺激RNA聚合酶III依赖性转录。
DOI：
10.1186/1750-2187-2-5
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发表时间：
2007-07-05
影响因子：
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通讯作者：
Chen, Shiuan
中科院分区：
文献类型：
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作者：
Zhou, Dujin;Zhong, Shuping;Ye, Jing-Jing;Quach, Keith M;Johnson, Deborah L;Chen, Shiuan
PNRC通过作为核受体辅激活剂转录调节广泛的RNA聚合酶（pol）II转录基因。为了寻找除核受体外的其他PNRC相互作用蛋白，将PNRC片段用作酵母双杂交筛选人乳腺cDNA表达文库的诱饵。RNA聚合酶III/RPC 39片段被反复确定为PNR C相互作用的合作伙伴在两个独立的筛选。这些RPC 39片段与PNRC之间的相互作用在独立的酵母双杂交试验中得到进一步证实。通过免疫共沉淀证明了MCF 7细胞中内源性PNRC和RPC 39的缔合。此外，ChIP分析检测到PNRC和RPC 39共募集到tRNA和U6 RNA启动子。进一步研究了PNRC和RPC 39之间相互作用的生物学后果。通过瞬时或稳定转染过表达PNRC，增加MCF 7细胞中RNA pol III依赖性转录，而观察到用PNRC/siRNA处理的MCF 7细胞中的转录减少。在这里，我们表明，人类PNRC刺激RNA聚合酶III的转录，通过其与RNA聚合酶III的亚基RPC 39的相互作用。PNRC是一种独特的辅激活因子，通过直接影响RNA pol II和RNA pol III依赖性转录，对细胞功能的许多方面产生深远影响。
PNRC transcriptionally regulates a wide range of RNA polymerase (pol) II-transcribed genes by functioning as a nuclear receptor coactivator. To search for additional PNRC-interacting proteins other than nuclear receptors, a PNRC fragment was used as bait in a yeast two-hybrid screening of a human mammary gland cDNA expression library. RNA pol III/RPC39 fragments were repeatedly identified as PNRC-interacting partners in two independent screenings. The interaction between these RPC39 fragments and PNRC was further confirmed in the independent yeast two-hybrid assays. The association of endogenous PNRC and RPC39 in MCF7 cells was demonstrated by co-immunoprecipitation. Furthermore, ChIP analysis detected co-recruitment of PNRC and RPC39 to tRNA and U6 RNA promoters. The biological consequence of the interaction between PNRC and RPC39 was further studied. Overexpression of PNRC, either by transient or stable transfection, increased RNA pol III-dependent transcription in MCF7 cells, while a decrease in transcription in MCF7 cells treated with PNRC/siRNA was observed. Here, we demonstrate that human PNRC stimulates RNA pol III transcription through its interaction with the subunit RPC39 of RNA pol III. PNRC is a unique coactivator that has profound effects on many aspects of cellular function by directly influencing both RNA pol II- and RNA pol III-dependent transcription.