Differentiation and maturation of zebrafish dorsal root and sympathetic ganglion neurons
Differentiation and maturation of zebrafish dorsal root and sympathetic ganglion neurons
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DOI：
10.1002/cne.10214
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发表时间：
2002-05-06
影响因子：
2.5
通讯作者：
Henion, PD
中科院分区：
文献类型：
--
作者：
An, M;Luo, RS;Henion, PD
脊椎动物胚胎的躯干神经嵴产生背根神经节（DRG）感觉神经元和自主交感神经元以及其他衍生物。我们研究了斑马鱼背根节和交感神经元在发育过程中的发育。我们发现，感觉神经元分化迅速，其明显的神经元分化显着领先于交感神经元在躯干。不同区域的交感神经元在不同的时间分化。最喙部的群体，我们称之为颈神经节，比躯干交感神经元早几天分化。在经历明显的神经元分化后，交感神经元随后表达肾上腺素能分化标志物多巴胺β-羟化酶和酪氨酸羟化酶。第二个群体的肾上腺素能非神经元细胞最初定位与颈交感神经元似乎代表肾上腺嗜铬细胞。在更成熟的鱼中，这些细胞在肾脏内成簇存在。单个DRG和交感神经节最初含有很少的神经元。然而，DRG和交感神经节中的神经元的数量至少在4周龄时持续增加。磷酸组蛋白H3表达和溴脱氧尿苷掺入研究的分析表明，DRG和交感神经节神经元细胞数量的增加是由于全部或部分神经节内的神经元细胞的分裂。(C)2002 Wiley-Liss，Inc.
The trunk neural crest of vertebrate embryos gives rise to dorsal root ganglion (DRG) sensory neurons and autonomic sympathetic neurons, among other derivatives. We have examined the development of DRG and sympathetic neurons during development in the zebrafish. We found that sensory neurons differentiate rapidly and that their overt neuronal differentiation significantly precedes that of sympathetic neurons in the trunk. Sympathetic neurons in different regions differentiate at different times. The most rostral population, which we call the cervical ganglion, differentiates several days before trunk sympathetic neurons. After undergoing overt neuronal differentiation, sympathetic neurons subsequently express the adrenergic differentiation markers dopamine beta-hydroxylase and tyrosine hydroxylase. A second population of adrenergic nonneuronal cells initially localized with cervical sympathetic neurons appears to represent adrenal chromaffin cells. In more mature fish, these cells were present in clusters within the kidneys. Individual DRG and sympathetic ganglia initially contain few neurons. However, the number of neurons in DRG and sympathetic ganglia increases continuously at least up to 4 weeks of age. Analysis of phosphohistone H3 expression and bromodeoxyuridine incorporation studies suggests that the increases in DRG and sympathetic ganglion neuronal cell number are due wholly or in part to the division of neuronal cells within the ganglia. (C) 2002 Wiley-Liss, Inc.