Estrogen and Luteinizing Hormone Secretion in Diverse Primate Species from Simplified Urinary Analysis
Estrogen and Luteinizing Hormone Secretion in Diverse Primate Species from Simplified Urinary Analysis
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简化尿液分析显示多种灵长类动物雌激素和黄体生成素的分泌
DOI：
10.1159/000460224
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发表时间：
1979
影响因子：
0.7
通讯作者：
B. Lasley
中科院分区：
文献类型：
--
作者：
J. Hodges;N. Czekala;B. Lasley
描述了监测各种灵长类动物尿液中雌激素和黄体生成素 (LH) 的实用方法。无需色谱法即可测量总免疫反应性雌激素 (Et) 水平，可以方便地估计雌酮、雌二醇和雌三醇作为其分离部分的总和。通过大鼠间质细胞睾酮 (RICT) 体外生物测定法测量尿液中的生物活性 LH。对尿肌酐含量进行校正，可以比较每日单次排尿收集的少量尿液的激素产生情况。使用这些方法，已经确定了人类、大猩猩、黑猩猩和卷尾猴生殖周期中的尿 Et 和 LH 谱。
A practical approach for monitoring urinary estrogen and luteinizing hormone (LH) in various primate species is described. Total immunoreactive estrogen (Et) levels, measured without chromatography, provided a convenient estimation of estrone, estradiol and estriol as the sum of their separated parts. Bioactive LH in urine was measured by the rat interstitial cell testosterone (RICT) in vitro bioassay. Correction for urinary creatinine content allowed for the comparison of hormone production from small volumes of urine collected from single daily voidings. Using these methods, urinary Et and LH profiles through the reproductive cycles of a human, gorilla, chimpanzee and capuchin monkey have been determined.