Epstein-Barr virus (P3HR-1) defective DNA codes for components of both the early antigen and viral capsid antigen complexes.
Epstein-Barr virus (P3HR-1) defective DNA codes for components of both the early antigen and viral capsid antigen complexes.
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Epstein-Barr 病毒 (P3HR-1) 编码早期抗原和病毒衣壳抗原复合物成分的 DNA 存在缺陷。
DOI：
10.1016/0042-6822(84)90003-5
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发表时间：
1984
期刊：
影响因子：
3.7
通讯作者：
Hayward,SD
中科院分区：
文献类型：
--
作者：
Cho,MS;Gissmann,L;Hayward,SD
从P3HR-1-超感染的Raji细胞中克隆了一组含有EB病毒（EBV）缺陷DNA重叠片段的λ基因。使用这些克隆的DNA片段获得的作图数据证实了先前直接从病毒体DNA推断的P3HR-1缺陷DNA的结构（M. - S.周湾，澳-地W. Bornkamm和H. zur豪森，1984，病毒学杂志，印刷中）。用间接免疫荧光试验检测克隆的缺陷DNA片段在转染的幼仓鼠肾（BHK）细胞中诱导EBV抗原的能力。在接受缺陷DNA片段BamHI-W ′ C ′的细胞中，高达5%的细胞瞬时表达新生儿抗原，通过其与特征性EBV阳性人血清的反应性，将其鉴定为EAD复合物的组分。发现P3HR-1缺陷DNA（EcoRI-C1）的20-kb克隆诱导VCA复合物的组分的合成。当用VCA+人血清或EBV抗VCA单克隆抗体检测时，用该克隆转染的细胞中有1%显示胞质荧光。EcoRI-C1片段的亚克隆将VCA基因定位于标准基因组的BamHI-A片段内的4.1-kb片段。该片段包含一个2.6 kb的单个大开放阅读框（B. Barrell，A.班基尔河Baer，P. Biggin，P. Deininger，P. Farrell，T.吉布森，G. Hatfull，G.哈德逊，S. Stachweli和C.金，1984，自然（伦敦），出版中）。没有缺陷DNA克隆能够诱导EBV特异性核抗原（EBNA），这与缺陷基因组中不存在已知的EBNA编码区一致。
A set of lambda phages containing overlapping fragments of Epstein-Barr virus (EBV) defective DNA has been cloned from P3HR-1-superinfected Raji cells. Mapping data obtained using these cloned DNA fragments confirmed the structure of P3HR-1 defective DNA previously deduced directly from virion DNA (M.-S. Cho, G. W. Bornkamm, and H. zur Hausen, 1984,J. Virol., in press). The ability of the cloned defective DNA fragments to induce EBV antigens in transfected baby hamster kidney (BHK) cells was tested using indirect immunofluorescence assays. Up to 5% of those cells receiving a defective DNA fragmentBamHI-W′C′ transiently expressed ade novonuclear antigen which was identified as being a component of the EADcomplex by its reactivity with characterized EBV-positive human sera. A 20-kb clone of P3HR-1 defective DNA (EcoRI-C1) was found to induce the synthesis of a component of the VCA complex. One percent of cells transfected with this clone showed cytoplasmic fluorescence when tested with either VCA+human sera or EBV anti-VCA monoclonal antibody. Subcloning of theEcoRI-C1 fragment localized the VCA gene to a 4.1-kb segment which maps within theBamHI-A fragment of the standard genome. This segment contains a single large open reading frame of 2.6 kb (B. Barrell, A. Bankier, R. Baer, P. Biggin, P. Deininger, P. Farrell, T. Gibson, G. Hatfull, G. Hudson, S. Stachweli, and C. Sequin, 1984,Nature (London), in press). None of the defective DNA clones were capable of inducing EBV-specific nuclear antigens (EBNAs) which is consistent with the absence of the known EBNA coding regions from the defective genome.