Primary and secondary structure of U8 small nuclear RNA.
Primary and secondary structure of U8 small nuclear RNA.
复制标题
U8小核RNA的一级和二级结构。
DOI：
10.1016/s0021-9258(17)39124-x
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
H. Busch
中科院分区：
文献类型：
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作者：
R. Reddy;D. Henning;H. Busch
U8小核RNA是在诺维科夫肝癌细胞中发现的一种新的、带帽的、140个核苷酸长的RNA。它的顺序是:m3GpppAmUmCGUCAGGA GGUUAAUCCU UACCUGUCCC UCCUUUCGGA GGGCAGAUAG AAAAUGAUGA UUGGAGCUUG CAUGAUCUGC UGAUUAUAGC AUUUCCGUGU AAUCAGGACC UGACAACAUC CUGAUUGCUU CUAUCUGAUUOH。这种RNA存在于大约25,000个拷贝/细胞中，并且在核仁制备中富集。与U1、U2、U4/U6和U5 RNA一样，U8 RNA也作为与Sm抗原相关的核糖核蛋白存在。大鼠U8 RNA高度同源（大于90%），与最近从感染脾脏病灶形成病毒的小鼠细胞中发现的5.4 S RNA高度同源（Kato， N., and Harada， F. (1984) Biochim）。Biophys。学报，782,127-131)。除了U8 RNA外，在标记大鼠和HeLa细胞的抗sm抗体免疫沉淀中还发现了另外三个U小核RNA。每一个都包含一个m3GpppAm帽结构；它们的表观链长分别为60、130和65个核苷酸。这U个小核rna分别被命名为U7、U9和U10 rna。
U8 small nuclear RNA is a new, capped, 140 nucleotides long RNA species found in Novikoff hepatoma cells. Its sequence is: m3GpppAmUmCGUCAGGA GGUUAAUCCU UACCUGUCCC UCCUUUCGGA GGGCAGAUAG AAAAUGAUGA UUGGAGCUUG CAUGAUCUGC UGAUUAUAGC AUUUCCGUGU AAUCAGGACC UGACAACAUC CUGAUUGCUU CUAUCUGAUUOH. This RNA is present in approximately 25,000 copies/cell, and it is enriched in nucleolar preparations. Like U1, U2, U4/U6, and U5 RNAs, U8 RNA was also present as a ribonucleoprotein associated with the Sm antigen. The rat U8 RNA was highly homologous (greater than 90%) to a recently characterized 5.4 S RNA from mouse cells infected with spleen focus-forming virus (Kato, N., and Harada, F. (1984) Biochim. Biophys. Acta, 782, 127-131). In addition to the U8 RNA, three other U small nuclear RNAs were found in anti-Sm antibody immunoprecipitates from labeled rat and HeLa cells. Each of these contained a m3GpppAm cap structure; their apparent chain lengths were 60, 130, and 65 nucleotides. These U small nuclear RNAs are designated U7, U9, and U10 RNAs, respectively.