Depth-sensitive diffuse speckle contrast topography for high-density mapping of cerebral blood flow in rodents.
Depth-sensitive diffuse speckle contrast topography for high-density mapping of cerebral blood flow in rodents.
复制标题
DOI：
10.1117/1.nph.10.4.045007
复制
发表时间：
2023-10
期刊：
影响因子：
5.3
通讯作者：
Yu, Guoqiang
中科院分区：
文献类型：
--
作者：
Mohtasebi, Mehrana;Singh, Dara;Liu, Xuhui;Fathi, Faraneh;Haratbar, Samaneh Rabienia;Saatman, Kathryn E.;Chen, Lei;Yu, Guoqiang
关键词：
脑血流量（CBF）的频繁评估是脑血管疾病的诊断和治疗的关键。与大型和昂贵的成像方式，如核医学和磁共振成像相比，光学成像技术是用于连续测量脑血流动力学的便携式和廉价的工具。最近开发的一种创新的非接触散斑对比度扩散相关断层扫描（scDCT），使三维（3D）成像的CBF分布。然而，scDCT需要复杂且耗时的3D重建，这限制了其在不牺牲时间分辨率和计算效率的情况下实现高空间分辨率的能力。我们研究了一种新的扩散散斑对比度地形（DSCT）方法与并行计算分析scDCT数据，以实现快速和高密度的二维（2D）映射CBF分布在不同的深度，而不需要三维重建。为了提高DSCT的采样率，采用了一种新的移动窗口方法。开发了一种利用图像处理软件和并行计算软件中MATLAB函数的快速计算方法，以快速生成高密度CBF图。新的DSCT方法的空间分辨率和深度灵敏度进行了测试，在头部模拟分层幻影和体内啮齿动物模型。DSCT能够通过不同厚度的顶层在不同深度处对体模中的粒子流进行2D映射。DSCT和scDCT都可以检测成年大鼠脑深部的整体和局部CBF变化。然而，DSCT实现了不同深度处CBF分布的快速和高密度2D映射，而不需要复杂和耗时的3D重建。深度敏感的DSCT方法有可能被用作一种非侵入性，非接触，快速，高分辨率，便携式和廉价的脑成像的基础神经科学研究在小动物模型和人类新生儿的转化研究。
Frequent assessment of cerebral blood flow (CBF) is crucial for the diagnosis and management of cerebral vascular diseases. In contrast to large and expensive imaging modalities, such as nuclear medicine and magnetic resonance imaging, optical imaging techniques are portable and inexpensive tools for continuous measurements of cerebral hemodynamics. The recent development of an innovative noncontact speckle contrast diffuse correlation tomography (scDCT) enables three-dimensional (3D) imaging of CBF distributions. However, scDCT requires complex and time-consuming 3D reconstruction, which limits its ability to achieve high spatial resolution without sacrificing temporal resolution and computational efficiency. We investigate a new diffuse speckle contrast topography (DSCT) method with parallel computation for analyzing scDCT data to achieve fast and high-density two-dimensional (2D) mapping of CBF distributions at different depths without the need for 3D reconstruction. A new moving window method was adapted to improve the sampling rate of DSCT. A fast computation method utilizing MATLAB functions in the Image Processing Toolbox™ and Parallel Computing Toolbox™ was developed to rapidly generate high-density CBF maps. The new DSCT method was tested for spatial resolution and depth sensitivity in head-simulating layered phantoms and in-vivo rodent models. DSCT enables 2D mapping of the particle flow in the phantom at different depths through the top layer with varied thicknesses. Both DSCT and scDCT enable the detection of global and regional CBF changes in deep brains of adult rats. However, DSCT achieves fast and high-density 2D mapping of CBF distributions at different depths without the need for complex and time-consuming 3D reconstruction. The depth-sensitive DSCT method has the potential to be used as a noninvasive, noncontact, fast, high resolution, portable, and inexpensive brain imager for basic neuroscience research in small animal models and for translational studies in human neonates.