NEUTROPHIL TETHERING TO AND ROLLING ON E-SELECTIN ARE SEPARABLE BY REQUIREMENT FOR L-SELECTIN
NEUTROPHIL TETHERING TO AND ROLLING ON E-SELECTIN ARE SEPARABLE BY REQUIREMENT FOR L-SELECTIN
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DOI：
10.1016/1074-7613(94)90107-4
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发表时间：
1994-05-01
期刊：
影响因子：
32.4
通讯作者：
SPRINGER, TA
中科院分区：
文献类型：
--
作者：
LAWRENCE, MB;BAINTON, DF;SPRINGER, TA
中性粒细胞在剪切流动中的束缚和滚动是由选择素介导的，并且被认为是选择素和配体之间单分子相互作用的两种难以区分的表现。然而，我们报告说，在生理流动条件下，粘附到e -选择素需要一个与支持中性粒细胞滚动的配体不同的配体。剪切拴在e -选择素上需要一个与中性粒细胞表面l -选择素的凝集素结构域密切相关的碳水化合物配体，因为l -选择素的酶切去除、趋化因子诱导的l -选择素脱落和l -选择素单克隆抗体有效地阻止了拴在e -选择素上。相比之下，这种配体对于在e -选择素上滚动的能力是必不可少的，因为静态孵育后形成的滚动粘附不受l -选择素存在与否的影响。因此，在l -选择素脱落后，e -选择素与中性粒细胞上的配体的相互作用仍然存在。这些发现为炎症部位白细胞定位的调控又增加了一步。
Neutrophil tethering and rolling in shear flow are mediated by selectins and have been thought to be two indistinguishable manifestations of a single molecular interaction between selectin and ligand. However, we report that under physiologic flow conditions, tethering to E-selectin requires a ligand distinct from the one that supports neutrophil rolling. Tethering under shear to E-selectin requires a carbohydrate ligand that is closely associated with the lectin domain of L-selectin on the neutrophil surface, as enzymatic removal of L-selectin, chemotactic factor-induced shedding of L-selectin, and L-selectin MAbs effectively block tethering. In contrast, this ligand is dispensable for the ability to roll on E-selectin, since rolling adhesions formed after static incubations were not affected by the presence or absence of L-selectin. Thus, E-selectin interactions with ligands on neutrophils persist after L-selectin shedding. These findings add an additional step for regulation of leukocyte localization in inflammatory sites.