Sae is essential for expression of the staphylococcal adhesins Eap and Emp
Sae is essential for expression of the staphylococcal adhesins Eap and Emp
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DOI：
10.1099/mic.0.27902-0
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发表时间：
2005-06-01
期刊：
影响因子：
2.8
通讯作者：
Herrmann, M
中科院分区：
文献类型：
--
作者：
Harraghy, N;Kormanec, J;Herrmann, M
EAP和EMP是两种金黄色葡萄球菌粘附素，最初被描述为细胞外基质结合蛋白。自那以后，EAP在真核细胞的黏附和侵袭中发挥了重要作用，并被描述为慢性感染的免疫调节剂和毒力因子。本文描述了EAP和EMP启动子转录起始点的定位。此外，利用报告基因分析和实时定量聚合酶链式反应，研究了环境条件和全球调控因子对EAP和emp表达的影响。纽曼株EAP和EMP的表达与8325的SH1000和8325-4有显著差异。此外，这两个基因在葡萄糖存在的情况下都受到抑制。对这两个基因在不同调控突变体中的表达分析表明，SarA和agR参与了它们的调控，但数据表明，这两个基因都有额外的调控因子。在一个sae突变体中，这两个基因的表达都被严重抑制。在葡萄糖存在的情况下，SAE的表达也被降低，这表明在含葡萄糖的介质中抑制EAP和EMP可能部分是SAE表达减少的结果。
Eap and Emp are two Staphylococcus aureus adhesins initially described as extracellular matrix binding proteins. Eap, has since emerged as being important in adherence to and invasion of eukaryotic cells, as well as being described as an immunomodulator and virulence factor in chronic infections. This paper describes the mapping of the transcription start point of the eap and emp promoters. Moreover, using reporter-gene assays and real-time PCR in defined regulatory mutants, environmental conditions and global regulators affecting expression of eap and emp were investigated. Marked differences were found in expression of eap and emp between strain Newman and the 8325 derivatives SH1000 and 8325-4. Moreover, both genes were repressed in the presence of glucose. Analysis of expression of both genes in various regulatory mutants revealed that sarA and agr were involved in their regulation, but the data suggested that there were additional regulators of both genes. In a sae mutant, expression of both genes was severely repressed. sae expression was also reduced in the presence of glucose, suggesting that repression of eap and emp in glucose-containing medium may, in part, be a consequence of a decrease in expression of sae.