Cas9-based genome editing in Xenopus tropicalis.
Cas9-based genome editing in Xenopus tropicalis.
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DOI：
10.1016/b978-0-12-801185-0.00017-9
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发表时间：
2014
影响因子：
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通讯作者：
Grainger, Robert M.
中科院分区：
文献类型：
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作者：
Nakayama, Takuya;Blitz, Ira L.;Fish, Margaret B.;Odeleye, Akinleye O.;Manohar, Sumanth;Cho, Ken W. Y.;Grainger, Robert M.
热带爪蟾已被开发为发育生物学的模式生物，提供了一个系统，提供现代遗传学和经典胚胎学。最近，用于基因组修饰的规则间隔短回文重复序列/CRISPR相关（CRISPR/Cas）系统为非洲爪蟾研究人员实现简单有效的靶向诱变提供了额外的工具。在这里，我们提供的实验设计和程序，允许成功应用这种技术的爪蟾研究人员的见解，并提供了一个通用的策略进行F0和随后的F1胚胎的功能丧失测定。
Xenopus tropicalis has been developed as a model organism for developmental biology, providing a system offering both modern genetics and classical embryology. Recently, the Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated (CRISPR/Cas) system for genome modification has provided an additional tool for Xenopus researchers to achieve simple and efficient targeted mutagenesis. Here, we provide insights into experimental design and procedures permitting successful application of this technique to Xenopus researchers, and offer a general strategy for performing loss-of-function assays in F0 and subsequently F1 embryos.