Selective induction of LTP and LTD by postsynaptic [Ca2+], elevation
Selective induction of LTP and LTD by postsynaptic [Ca2+], elevation
复制标题
DOI：
10.1152/jn.1999.81.2.781
复制
发表时间：
1999-02-01
影响因子：
2.5
通讯作者：
Zucker, RS
中科院分区：
文献类型：
--
作者：
Yang, SN;Tang, YG;Zucker, RS
长期增强（LTP）和长期抑制（LTD）是CA1海马锥体细胞谷氨酸能传入神经突触可塑性的两种主要形式，都是由突触后细胞内钙浓度（[Ca2+](i)）的升高触发的。为了了解一个信号分子如何负责触发两种相反形式的突触调节，在CA1锥体细胞中，一种新的笼化钙化合物硝基苯基-乙二醇-双（β -氨基乙醚）-N，N，N‘，N’-四乙酸产生了不同的突触后[Ca2+](i)升高模式。我们发现特定模式的[Ca2+](i)升高选择性地激活LTP或LTD。特别是，只有LTP是由[Ca2+](i)的短暂增加和相对较高的幅度触发的，这模仿了通常用于诱导LTP的电刺激期间的[Ca2+](i)上升。相比之下，[Ca2+](i)的长期适度上升可靠地诱导LTD。该结果的一个重要含义是，细胞内化学信号的振幅和持续时间都可以携带重要的生物信息。
Long-term potentiation (LTP) and long-term depression (LTD), two prominent forms of synaptic plasticity at glutamatergic afferents to CA1 hippocampal pyramidal cells, are both triggered by the elevation of postsynaptic intracellular calcium concentration ([Ca2+](i)). To understand how one signaling molecule can be responsible for triggering two opposing forms of synaptic modulation, different postsynaptic [Ca2+](i) elevation patterns were generated by a new caged calcium compound nitrophenyl-ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid in CA1 pyramidal cells. We found that specific patterns of [Ca2+](i) elevation selectively activate LTP or LTD. In particular, only LTP was triggered by a brief increase of [Ca2+](i) with relatively high magnitude, which mimics the [Ca2+](i) rise during electrical stimulation typically used to induce LTP. In contrast, a prolonged modest rise of [Ca2+](i) reliably induced LTD. An important implication of the results is that both the amplitude and the duration of an intracellular chemical signal can carry significant biological information.