Immunohistochemical studies on EGF family growth factors in normal and ulcerated human gastric mucosa
Immunohistochemical studies on EGF family growth factors in normal and ulcerated human gastric mucosa
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DOI：
10.1023/a:1018897922644
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发表时间：
1997-06-01
影响因子：
3.1
通讯作者：
Toyota, T
中科院分区：
文献类型：
--
作者：
Abe, S;Sasano, H;Toyota, T
通过免疫组织化学方法研究了表皮生长因子家族成员（包括表皮生长因子 (EGF)、转化生长因子-α (TGF-α)、双调蛋白 (AR) 和 Cripto）及其假定受体表皮生长因子受体 (EGFR) 在人胃粘膜中的表达，以评估它们在正常和再生胃粘膜细胞增殖中的可能作用。我们还通过增殖细胞核抗原（PCNA）和 EGFR 的双重免疫组织化学染色检查了细胞增殖和 EGFR 之间的相关性。在正常胃粘膜中，胃底腺表面上皮细胞和壁细胞分别观察到TGF-α、Cripto和AR免疫反应性。在所检查的任何正常粘膜中均未观察到 EGF 免疫反应性。在增殖区的小凹细胞和壁细胞中检测到EGFR免疫反应性，双重免疫染色显示EGFR免疫反应性比PCNA免疫反应性分布更广泛。邻近胃溃疡边缘的PCNA阳性上皮细胞表达相对强烈的EGFR，但不表达任何所检查的生长因子。另一方面，在远离胃溃疡边缘的 PCNA 阴性再生上皮中检测到相对强烈的 TGF-α 和 Cripto 免疫反应性。与溃疡相关的再生胃上皮中AR的相对免疫反应性与正常胃粘膜中的相对免疫反应性没有不同。 TGF-α、AR 和 Cripto 被认为在正常胃粘膜增殖中发挥重要作用，并且 TGF-α 和 Cripto 可能通过旁分泌机制参与溃疡愈合。
Expression of members of the epidermal growth factor family, including epidermal growth factor (EGF), transforming growth factor-alpha (TGF-alpha), amphiregulin (AR), and Cripto, as well as their putative receptor, epidermal growth factor receptor (EGFR), was studied immunohistochemically in human gastric mucosa to evaluate their possible roles in cell proliferation of normal and regenerative gastric mucosa. We also examined the correlation between cell proliferation and EGFR by double immunohistochemical staining for proliferating cell nuclear antigen (PCNA) and EGFR. In normal gastric mucosa, TGF-alpha, Cripto, and AR immunoreactivities were observed in the surface epithelial and parietal cells of gastric fundic glands, respectively. EGF immunoreactivity was not observed in any of normal mucosa examined. EGFR immunoreactivity was detected on foveolar cells in proliferative zones and in parietal cells, Double immunostaining revealed that EGFR immunoreactivity was distributed much more widely than PCNA immunoreactivity. PCNA positive epithelial cells adjacent to gastric ulcer margin expressed relatively intense EGFR but did not express any of the growth factors examined. On the other hand, relatively intense immunoreactivity of both TGF-alpha and Cripto was detected in PCNA-negative regenerative epithelium located distant from gastric ulcer margin. Relative immunoreactivity of AR in regenerative gastric epithelium associated with ulcer was not different from that in normal gastric mucosa. TGF-alpha, AR, and Cripto are considered to play important roles in normal gastric mucosal proliferation, and TGF-alpha and Cripto may be involved in ulcer healing, possibly via a paracrine mechanism.