Expression of growth hormone gene in the baboon eye
Expression of growth hormone gene in the baboon eye
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DOI：
10.1016/j.exer.2018.01.002
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发表时间：
2018-04-01
影响因子：
3.4
通讯作者：
Barrera-Saldana, Hugo Alberto
中科院分区：
文献类型：
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作者：
Perez-Ibave, Diana Cristina;Rodriguez-Sanchez, Iram Pablo;Barrera-Saldana, Hugo Alberto
人类生长激素 (GH) 基因座由两个 GH（GH1 和 GH2）基因和三个绒毛膜 somatomanunoIropin（CSH1、CSH2 和 CSH-L）基因组成。 GHI 在垂体中表达，其余则在胎盘中表达。然而，GH1 也在多种垂体外组织中表达，包括眼睛。因此，为了了解这种激素在眼睛中的作用，我们使用了狒狒 (Papio hmnathyas)，它像人类一样具有多基因 GH 基因座；我们旨在研究狒狒成年和胎儿眼组织中GH位点的表达和调控。我们在狒狒眼组织中检索了GH1、GH2、CSH1/2、Pitl（垂体转录因子1）、GHR（生长激素受体）、GHRH（生长激素释放激素）、GHRHR（生长激素释放激素受体）、SST（生长抑素）、SSTRI（生长抑素受体）的表达1)、SSTR2（生长抑素受体 2）、SSTR3（生长抑素受体 3）、SSTR4（生长抑素受体 4）和 SSTR5（生长抑素受体 5）mRNA 转录物和衍生蛋白，分别通过 qPCR 和免疫荧光测定。对所发现的转录本进行了cDNA克隆和测序鉴定，仅发现了属于GHI基因的转录本，主要存在于视网膜/脉络膜组织中。通过免疫荧光测定，在多个视网膜细胞层中证实了 GH1 和 GHR 蛋白的存在。可能控制局部 GH1 表达的神经内分泌调节因子包括 GHRH 和 SST，因为它们的 mRNA5 和蛋白质主要存在于视网膜/脉络膜组织中，以及它们相应的受体（GHRH 和 SSTR1-SSTR5）。眼部组织均不表达 Pitl，因此狒狒眼中 GHI 基因的表达可能独立于 Pitl。我们得出的结论是，为了了解人眼中 GH 的调节，狒狒提供了一个非常好的实验模型。
The human growth hormone (GH) locus is comprised by two GH (GH1 and GH2) genes and three chorionic somatomanunoIropin (CSH1, CSH2 and CSH-L) genes. While GHI is expressed in the pituitary gland, the rest are expressed in the placenta. However, GH1 is also expressed in several extrapituitary tissues, including the eye. So to understand the role of this hormone in the eye we used the baboon (Papio hmnathyas), that like humans has a multigenic GH locus; we set up to investigate the expression and regulation of GH locus in adult and fetal baboon ocular tissues.We searched in baboon ocular tissues the expression of GH1, GH2, CSH1/2, Pitl (pituitary transcription factor 1), GHR (growth hormone receptor), GHRH (growth hormone releasing hormone), GHRHR (growth hormone releasing hormone receptor), SST (somatostatin), SSTRI (somatostatin receptor 1), SSTR2 (somatostatin receptor 2), SSTR3 (somatostatin receptor 3), SSTR4 (somatostatin receptor 4), and SSTR5 (somatostatin receptor 5) mRNA transcripts and derived proteins, by qPCR and immunofluorescence assays, respectively. The transcripts found were characterized by cDNA cloning and sequencing, having found only the one belonging to GHI gene, mainly in the retina/choroid tissues. Through immunofluorescence assays the presence of GH1 and GHR proteins was confirmed in several retinal cell layers. Among the possible neuroendocrine regulators that may control local GH1 expression are GHRH and SST, since their mRNA5 and proteins were found mainly in the retina/choroid tissues, as well as their corresponding receptors (GHRH and SSTR1-SSTR5). None of the ocular tissues express Pitl, so gene expression of GHI in baboon eye could be independent of Pitl. We conclude that to understand the regulation of GH in the human eye, the baboon offers a very good experimental model.