Microbial eukaryotes in the suboxic chemosynthetic ecosystem of Movile Cave, Romania.
Microbial eukaryotes in the suboxic chemosynthetic ecosystem of Movile Cave, Romania.
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罗马尼亚莫维尔洞穴低氧化学合成生态系统中的微生物真核生物。
DOI：
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发表时间：
2019
影响因子：
3.3
通讯作者：
P. López‐García
中科院分区：
文献类型：
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作者：
G. Reboul;D. Moreira;P. Bertolino;Alexandra Hillebrand;P. López‐García
莫维尔洞穴是罗马尼亚东南部靠近黑海的石灰岩环境中部分被淹没的小洞穴系统。从地表分离了600万年，其富含硫化物、甲烷和氨的水域孕育着独特的化学合成原核生物群落，包括硫和氨代谢的化学养石菌、产甲烷菌、甲烷营养菌和甲基营养菌。洞穴中还栖息着穴居无脊椎动物和真菌，但其他真核微生物的多样性仍然完全未知。本文采用基于18S rRNA基因的元条形码方法，研究了一个保存完好的缺氧洞穴中浮游微生物垫和浮游生物的原生群落组成。我们的研究结果揭示了广泛的原生生物多样性，作为优势类群，纤毛虫（Alveolata），层虫（尤其是双孔虫）和jakobids （Excavata）。纤毛虫序列占主导地位，微生物席和浮游生物，其次是层桩或挖掘。叠层虫在微生物席中更为突出，而jakobids在缺氧水柱中的浮游生物部分占主导地位。在实验室培养的草席富含尾藻。与当地低氧水平一致，莫维尔洞穴原生生物很可能是厌氧或嗜微气的。一些新发现的OTU分支与数据库中培养的物种或环境序列非常不同，并且在系统发育上具有新新性，特别是在jakobids中。Movile洞穴原生生物可能在这个生态系统中扮演着多种生态角色，包括捕食、寄生、腐坏和可能的多种原核-原生生物同质。
Movile Cave is a small system of partially inundated galleries in limestone settings close to the Black Sea in Southeast Romania. Isolated from the surface for 6 million years, its sulfidic, methane and ammonia-rich waters harbour unique chemosynthetic prokaryotic communities that include sulphur and ammonium-metabolizing chemolithotrophs, methanogens, methanotrophs and methylotrophs. The cave also harbours cave-dwelling invertebrates and fungi, but the diversity of other microbial eukaryotes remained completely unknown. Here, we apply an 18S rRNA gene-based metabarcoding approach to study the composition of protist communities in floating microbial mats and plankton from a well-preserved oxygen-depleted cave chamber. Our results reveal a wide protist diversity with, as dominant groups, ciliates (Alveolata), Stramenopiles, especially bicosoecids, and jakobids (Excavata). Ciliate sequences dominated both, microbial mats and plankton, followed by either Stramenopiles or excavates. Stramenopiles were more prominent in microbial mats, whereas jakobids dominated the plankton fraction of the oxygen-depleted water column. Mats cultured in the laboratory were enriched in Cercozoa. Consistent with local low oxygen levels, Movile Cave protists are most likely anaerobic or microaerophilic. Several newly detected OTU clades were very divergent from cultured species or environmental sequences in databases and represent phylogenetic novelty, notably within jakobids. Movile Cave protists likely cover a variety of ecological roles in this ecosystem including predation, parasitism, saprotrophy and possibly diverse prokaryote-protist syntrophies.
DOI：
10.1038/ismej.2014.102
发表时间：
2014-07
期刊：
The ISME Journal
影响因子：
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作者：
D. Wischer;D. Kumaresan;D. Kumaresan;A. Johnston;Myriam El Khawand;Jason Stephenson;Alexandra Hillebrand-Voiculescu;Yin Chen;J. Murrell
通讯作者：
D. Wischer;D. Kumaresan;D. Kumaresan;A. Johnston;Myriam El Khawand;Jason Stephenson;Alexandra Hillebrand-Voiculescu;Yin Chen;J. Murrell